Presto™ Plasmid DNA Concentration Kit
DNA Extraction/Plasmid DNA Purification
The Presto™ Plasmid DNA Concentration Kit offers a simplified replacement for time-consuming plasmid DNA precipitation procedures. The Presto™ Plasmid Concentrator will quickly and efficiently concentrate plasmid DNA in anion-exchange chromatographic DNA purification eluates collected from Geneaid Plasmid Midi Columns, Macherey-Nagel NucleoBond® AX, NucleoBond® Xtra Columns, QIAGEN® tip 500 Columns etc. Plasmid eluates are precipitated with isopropanol then passed through a Presto™ Plasmid Concentrator using a syringe. Once contaminants are removed using a proprietary Desalting Buffer, the concentrator is dried and plasmid DNA is eluted with a small volume (100-400 μl) of low salt Elution Buffer. The Presto™ Plasmid Concentrator eliminates DNA pellet loss and incomplete solubilization of precipitates to ensure highly concentrated, purified plasmid DNA. In only 10 minutes, the plasmid DNA can be used directly in a variety of sensitive downstream applications, such as transfection, sequencing, ligation, PCR, in-vitro transcription, microinjection, restriction enzyme digestion and gene gun.
Specifications (Cat. # PC0250/251, PC0500/501, PC1000/1001)
- Concentrate plasmid DNA in only 10 minutes compared to 60 minutes using traditional plasmid DNA precipitation!
- Convenient: replaces traditional, time-consuming plasmid DNA precipitation procedures
- Binding Capacity: 600 µg of plasmid DNA
- Recovery: up to 90%
- Concentration: up to 3.2 µg/µl
- Plasmid Size: 1-20 kb
- Elution Volume: 100-400 µl
- Dead Volume: 25 µl
- Operation Time: 10 minutes (manual), 20 minutes/6 preps (vacuum)
- Storage: dry at room temperature (15-25°C)
Applications
Transfection, sequencing, ligation, PCR, in-vitro transcription, microinjection, restriction enzyme digestion and gene gun
Components
- Presto™ Plasmid Concentrator
- Desalting Buffer
- Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
- 20 ml syringe
- 1 ml syringe
Quality Control
The quality of the Presto™ Plasmid DNA Concentration Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system.
Presto™ Plasmid DNA Concentrator DNA Recovery and Concentration
Input DNA | Elution Volume | ||||
| 100 µl | 200 µl | 400 µl | 600 µl | ||
| 100 µg | Recovery | 65% | 80% | 90% | 90% |
| Concentration | 0.9 µg/µl | 0.45 µg/µl | 0.2 µg/µl | 0.15 µg/µl | |
| Eluate | 75 µl | 175 µl | 375 µl | 575 µl | |
| 300 µg | Recovery | 55% | 70% | 80% | 90% |
| Concentration | 2.2 µg/µl | 1.2 µg/µl | 0.65 µg/µl | 0.4 µg/µl | |
| Eluate | 75 µl | 175 µl | 375 µl | 575 µl | |
| 600 µg | Recovery | 40% | 60% | 75% | 75% |
| Concentration | 3.2 µg/µl | 2.1 µg/µl | 1.2 µg/µl | 0.8 µg/µl | |
| Eluate | 75 µl | 175 µl | 375 µl | 575 µl | |
Table 1.DNA Recovery is highly dependant on the volume of elution buffer used. 90% recovery can be acheived when using 600 μl of elution buffer. However, DNA concentration is decreased. When using small volumes of elution buffer, recovery is decreased but concentration is increased.
| RELATED PRODUCTS | ||
|---|---|---|
| Product Name | RXNS/VOLUME | Catalogue Number |
| Presto™ Endotoxin Free Mini Plasmid Kit | 100 | PEH100 |
| Presto™ Mini Plasmid Kit | 100, 300 | PDH100/300 |
| High-Speed Plasmid Mini Kit | 100, 300 | PD100/300 |
| Large Plasmid DNA Extraction Kit | 100, 300 | PDL100/300 |
| Midiprep Spin Column Plasmid Kit | 25 | PA025 |
| Presto™ Midi Plasmid Kit | 25 | PIF025 |
| Presto™ Midi Plasmid Kit (Endotoxin Free) | 25 | PIFE25 |
| Geneaid™ Midi Plasmid Kit | 25 | PI025 |
| Geneaid™ Midi Plasmid Kit (Endotoxin Free) | 25 | PIE25 |
| Presto™ Plasmid DNA Concentration Kit | 25, 50, 100 | PC0250/500/1000 |
| Geneaid™ Maxi Plasmid Kit | 10, 25 | PM010/25 |
| Geneaid™ Maxi Plasmid Kit (Endotoxin Free) | 10, 25 | PME10/25 |
| Presto™ 96 Well Plasmid Kit | 4 x 96, 10 x 96 | 96PDC04/10 96PDV04/10 |
