Geneaid™ Midi Plasmid Kit
DNA Extraction/Plasmid DNA Purification
The Geneaid™ Midi Plasmid Kit uses pre-packed anion-exchange resin columns to purify plasmid DNA from 50-300 ml of cultured bacterial cells. TrueBlue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. Using an efficient gravity-flow procedure, plasmid DNA is bound to the column and contaminants are efficiently removed. The purified plasmid DNA is eluted then precipitated with isopropanol for desalting. The entire procedure can be completed without ultracentrifuges, HPLC or other toxic reagents and the purified plasmid DNA is suitable for transfection, sequencing reactions, ligation, PCR, in-vitro transcription, microinjection, restriction enzyme digestion and gene gun.
Specifications (Cat. # PI025)
- High Yield: 350 µg of pure transfection grade plasmid DNA from 100 ml of cultured bacterial cells
- Sample Volume: 50-300 ml of cultured bacterial cells
- Format: plasmid midi anion-exchange resin column
- Elution Volume: 500 µl – 2 ml
- Operation time: within 80 minutes (only 50 minutes when combined with the Presto™ Plasmid Concentrator)
- Storage: dry at room temperature (15-25ºC)
Transfection, Sequencing, Ligation, PCR, In-vitro Transcription, Microinjection, Restriction Enzyme Digestion, Gene Gun
Components
- PM1, PM2, PM3 Buffer
- TrueBlue Lysis Buffer
- PEQ Buffer
- PMC Buffer
- PEL Buffer
- RNase A
- Plasmid Midi Columns
Quality Control
The quality of the Geneaid™ Midi Plasmid Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. Plasmid DNA from a 100 ml overnight E. coli (DH5α) culture, containing plasmid pBluescript (A600 > 2 U/ml) is purified using the Geneaid™ Midi Plasmid Kit. Following the purification process, a yield of more than 300 μg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.
Plasmid DNA Midi and Maxi Kits Specifications
| Specifications | Presto™ Midi Plasmid Kit | Presto™ Midi Plasmid Kit Endotoxin Free | Geneaid™ Midi Plasmid Kit | Geneaid™ Midi Plasmid Kit Endotoxin Free | Geneaid™ Maxi Plasmid Kit | Geneaid™ Maxi Plasmid Kit Endotoxin Free |
|---|---|---|---|---|---|---|
| Cat. # | PIF025 | PIFE25 | PI025 | PIE25 | PM010/25 | PME10/25 |
| Format | Anion-Exchange Gravity Flow | Anion-Exchange Gravity Flow | Anion-Exchange Gravity Flow | Anion-Exchange Gravity Flow | Anion-Exchange Gravity Flow | Anion-Exchange Gravity Flow |
| Binding Capacity | 350 µg | 350 µg | 350 µg | 350 µg | 1.0 mg | 1.0 mg |
| Filter Column | Yes | Yes | N/A | N/A | N/A | N/A |
| Endotoxin Level | <0.15 EU/µg | <0.10 EU/µg | <0.15 EU/µg | <0.10 EU/µg | <0.15 EU/µg | <0.10 EU/µg |
| Culture Input | 50-150 ml | 50-150 ml | 50-300 ml | 50-300 ml | 200-1600 ml | 200-1600 ml |
| Plasmid Size | 1-20 kb | 1-20 kb | 1-20 kb | 1-20 kb | 1-20 kb | 1-20 kb |
| Typical Yield | 350 µg | 350 µg | 350 µg | 350 µg | 1.0 mg | 1.0 mg |
| Elution Volume | 500 µl-2 ml | 500 µl-2 ml | 500 µl-2 ml | 500 µl-2 ml | 500 µl-2 ml | 500 µl-2 ml |
| Operation Time | <80 minutes | <80 minutes | <80 minutes | <80 minutes | <100 minutes | <100 minutes |
| RELATED PRODUCTS | ||
|---|---|---|
| Product Name | RXNS/VOLUME | Catalogue Number |
| Presto™ Endotoxin Free Mini Plasmid Kit | 100 | PEH100 |
| Presto™ Mini Plasmid Kit | 100, 300 | PDH100/300 |
| High-Speed Plasmid Mini Kit | 100, 300 | PD100/300 |
| Large Plasmid DNA Extraction Kit | 100, 300 | PDL100/300 |
| Midiprep Spin Column Plasmid Kit | 25 | PA025 |
| Presto™ Midi Plasmid Kit | 25 | PIF025 |
| Presto™ Midi Plasmid Kit (Endotoxin Free) | 25 | PIFE25 |
| Geneaid™ Midi Plasmid Kit | 25 | PI025 |
| Geneaid™ Midi Plasmid Kit (Endotoxin Free) | 25 | PIE25 |
| Presto™ Plasmid DNA Concentration Kit | 25, 50, 100 | PC0250/500/1000 |
| Geneaid™ Maxi Plasmid Kit | 10, 25 | PM010/25 |
| Geneaid™ Maxi Plasmid Kit (Endotoxin Free) | 10, 25 | PME10/25 |
| Presto™ 96 Well Plasmid Kit | 4 x 96, 10 x 96 | 96PDC04/10 96PDV04/10 |
