Presto™ Mini gDNA Bacteria Kit
DNA Extraction/Genomic DNA Purification
The Presto™ Mini gDNA Bacteria Kit is a Bacteria genomic DNA extraction kit optimized for genomic and viral DNA purification from Gram negative bacterial cells and Gram positive bacterial cells. This Bacteria Genomic DNA Extraction Kit includes Gram+ Buffer and Lysozyme and when combined, will efficiently lyse bacterial cell walls consisting of the peptidoglycan layer. Chaotropic salt is used to lyse cells and degrade protein, allowing DNA to easily bind to the glass fiber matrix of the genomic DNA spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 30 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA, with approximately 20-40 kb, is suitable for use in PCR or other enzymatic reactions.
Specifications (Cat. # GBB100, GBB101, GBB300, GBB301)
- Purify genomic DNA within 30 minutes!
- High Yield: up to 40 μg (1 x 109 Escherichia coli) and 15 µg (1 x 109 Bacillus subtilis)
- High Purity gDNA: A260/A280 >1.9
- DNA Size: 20-40 kb
- Samples: Gram (+) Positive and Gram (-) Negative bacterial cells (up to 1 x 109), 200 µl of blood and biological fluids such as plasma and urine
- Convenient: Includes Gram+ Buffer for preparing Lysozyme solutions to speed up sample preparation when purifying DNA from Gram (+) positive bacteria
- Spin Columns: glass fiber membrane optimized for genomic DNA extraction (sterilised to remove bacteria contamination)
- Elution volume: 30-200 µl
- Storage: dry at room temperature (15-25°C)
- PCR,
- Restriction Endonuclease Digestions,
- Array,
- Southern Blots and various other enzymatic reactions
Components
- Proteinase K
- Gram+ Buffer
- GT Buffer
- GB Buffer
- W1 Buffer
- Wash Buffer
- Lysozyme
- Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
- GD Columns
- 2 ml Collection Tubes
Quality Control
The quality of the Presto™ Mini gDNA Bacteria Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. The purified genomic DNA from Escherichia coli cells (up to 1 x 109), (up to 40 µg with an A260/A280 ratio of >2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.
Genomic DNA Extraction Kits Specifications
| Specifications | gSYNC™ DNA Extraction Kit | Genomic DNA Mini Kit Blood Cultured Cell | Genomic DNA Mini Kit Tissue | Genomic DNA Mini/Maxi Kit Plant | Presto™ Mini gDNA Bacteria Kit | Presto™ Mini gDNA Yeast Kit | Geneius™ Micro gDNA Extraction Kit | Presto™ Buccal Swab gDNA Extraction Kit | ||
| Cat. # | GS100 GS300 | GB100 GB300 | GT050 GT100 GT300 | GP100 GPM010 GPM025 | GBB100/101 GBB300/301 | GBYB100 GBYB300 | GMB100 GMB300 | GSK100 GSK300 | ||
| Format | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Reagent System | Spin Column | Spin Column | ||
| Binding Capacity | 30 µg | 30 µg | 30 µg | 50 µg (Mini) 500 µg (Maxi) | 50 µg | N/A | 50 µg | 50 µg | ||
| Sample | 200 µl blood | 300 µl blood | 30 mg animal tissue | Mini: 100 mg plant tissue | 1x109 bacterial cells | 2x108 yeast cells | 1-100 µl blood | buccal swabs | ||
| 25 mg animal tissue | 200 µl buffy coat | Maxi: 1 g plant tissue | 200 µl blood | FTA® Cards | ||||||
| 1x107 cultured cells | 1x107 cultured cells | 1 ml urine, plasma | 1 ml urine | |||||||
| hair | 2x108 yeast/fungus | 1-100 µl saliva | ||||||||
| Micropestle | N/A | N/A | Yes | N/A | N/A | N/A | N/A | N/A | ||
| Filter Column | N/A | N/A | N/A | Yes | N/A | N/A | N/A | Yes | ||
| Enzyme | Proteinase K | N/A | Proteinase K | N/A | Lysozyme Proteinase K | Lyticase or Zymolase | Proteinase K | Proteinase K | ||
| Typical Yield | 5 µg from 200 µl blood | 4-10 µg from 300 µl blood | 10-40 µg from 20 mg tissue | 3-5 µg from 100 mg Arabidopsis leaf (Mini) | 37 µg from 1x109 E. coli | 10-15 µg from 2x108 S. cerevisiae cells | 300 ng from 6 mm blood spots | 2 µg per swab | ||
| 10-40 µg from 20 mg tissue | 80 µg from 500 mg Lemon leaf (Maxi) | 10-15 µg from 1x109 Bacillus subtilis | ||||||||
| Elution Volume | 30-100 µl | 30-200 µl | 30-200 µl | 30-200 µl (Mini) 1 ml (Maxi) | 30-200 µl | 50-100 µl | 30-100 µl | 50-100 µl | ||
| Operation Time | <20 minutes | <25 minutes | 30 minutes | <30 minutes | <30 minutes | <40 minutes | <20 minutes | <20 minutes |
| Product Name | RXNS | Catalogue Number | ||
| gSYNC™ DNA Extraction Kit (Tissue/Blood) | 100, 300 | GS100/300 | ||
| Genomic DNA Mini Kit (Blood/Cultured Cell) | 100, 300 | GB100/300 | ||
| Genomic DNA Maxi Kit (Plant) | 10, 25 | GPM010/25 | ||
| Genomic DNA Maxi Kit (Blood/Cultured Cell) | 10, 25 | GDM10/25 | ||
| Genomic DNA Mini Kit (Tissue) | 50, 100, 300 | GT050/100/300 | ||
| Genomic DNA Mini Kit (Plant) | 100 | GP100 | ||
| Geneius™ Micro gDNA Extraction Kit | 100 | GMB100/300 | ||
| Presto™ Buccal Swab gDNA Extraction Kit | 100 | GSK100/300 | ||
| Presto™ Mini gDNA Yeast Kit | 100, 300 | GBYB100/300 | ||
| Presto™ Mini gDNA Bacteria Kit | 100, 300 | GBB100/101/300/301 | ||
| Presto™ 96 Well Blood Genomic DNA Extraction Kit | 4 x 96, 10 x 96 | 96GBP04/10 | ||
| Presto™ 96 Well Plant Genomic DNA Extraction Kit | 4 x 96, 10 x 96 | 96GPP04/10 | ||
| Presto™ 96 Well gDNA Bacteria Kit | 4 x 96, 10 x 96 | 96GBB04/10 |
