GENEzol™ TriRNA Bacteria Kit with DNase
RNA Extraction/Tri RNA
The GENEzol™ TriRNA Bacteria Kit is a phenol and guanidine isothiocyanate plus spin column system for convenient purification of high-quality total RNA from bacteria samples. Bacterial cell walls are initially lysed using Lysozyme. The sample is then homogenized in GENEzol™ Reagent without chloroform phase separation or isopropanol RNA precipitation. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of sensitive downstream applications.
Advantages (Cat. # GZBD050, GZBD100, GZBD200)
- Purify total RNA within 20 minutes without chloroform phase separation or isopropanol RNA precipitation
- Sample Volume: bacteria cells up to 1 x 109
- Format: phenol, chlorform, guanidine isothiocyanate plus spin column system
- High quality RNA: A260/A280 >1.8, A260/A230 >1.8
- Storage: GENEzol™ Reagent should be stored at 2ºC to 25ºC, DNase I and Lysozyme can be shipped at room temperature but should be stored at -20ºC for extended periods
- cDNA Library Construction,
- Cloning,
- RT-PCR (Endpoint),
- Real-Time PCR,
- Nuclease Protection Assays,
- Northern Blotting
Components
- GENEzol™ Reagent
- Pre-Wash Buffer
- Lysozyme
- Bacteria Lysis Buffer
- DNase I
- DNase Reaction Buffer
- Wash Buffer
- RNase-free Water
- RB Columns
- 2 ml Collection Tubes
Quality Control
The GENEzol™ TriRNA Bacteria Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. 5 µl from a 50 µl eluate of purified RNA is analyzed by electrophoresis on a 1% agarose gel.
| Product Name | RXNS | Catalogue Number |
|---|---|---|
| GENEzol™ Reagent | 50/100/200 | GZR050/100/200 |
| GENEzol™ TriRNA Pure Kit | 50/100/200 | GZX050/100/200, GZXD050/100/200 |
| GENEzol™ TriRNA Bacteria Kit | 50/100 | GZB050/100 |
| TriRNA Pure Kit | 50/100/200 | TRP050/100/200, TRPD050/100/200 |
| RNA Cleanup Kit | 50/100 | PR050/100 |
| GENEzol™ 96 Well TriRNA Pure Kit | 4 x 96/10 x 96 | 96GZX04/10 |
