The 96 Well gSYNC™ DNA Extraction Kit is optimized for high-throughput genomic DNA extraction from tissue and cells. This DNA extraction kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the 96 Well Binding Plate. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 60 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions.
The quality of the 96 Well gSYNC™ DNA Extraction Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. Genomic DNA from 200 μl of whole human blood is extracted using the gSYNC™ DNA Extraction Kit. The purified DNA (5 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.
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Specifications | gSYNC™ DNA Extraction Kit | Genomic DNA Mini Kit Blood Cultured Cell | Genomic DNA Mini Kit Tissue | Genomic DNA Mini/Maxi Kit Plant | Presto™ Mini gDNA Bacteria Kit | Presto™ Mini gDNA Yeast Kit | Geneius™ Micro gDNA Extraction Kit | Presto™ Buccal Swab gDNA Extraction Kit | ||
Cat. # | GS100 GS300 | GB100 GB300 | GT050 GT100 GT300 | GP100 GPM010 GPM025 | GBB100/101 GBB300/301 | GBYB100 GBYB300 | GMB100 GMB300 | GSK100 GSK300 | ||
Format | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Reagent System | Spin Column | Spin Column | ||
Binding Capacity | 30 µg | 30 µg | 30 µg | 50 µg (Mini) 500 µg (Maxi) | 50 µg | N/A | 50 µg | 50 µg | ||
Sample | 200 µl blood | 300 µl blood | 30 mg animal tissue | Mini: 100 mg plant tissue | 1x109 bacterial cells | 2x108 yeast cells | 1-100 µl blood | buccal swabs | ||
25 mg animal tissue | 200 µl buffy coat | Maxi: 1 g plant tissue | 200 µl blood | FTA® Cards | ||||||
1x107 cultured cells | 1x107 cultured cells | 1 ml urine, plasma | 1 ml urine | |||||||
hair | 2x108 yeast/fungus | 1-100 µl saliva | ||||||||
Micropestle | N/A | N/A | Yes | N/A | N/A | N/A | N/A | N/A | ||
Filter Column | N/A | N/A | N/A | Yes | N/A | N/A | N/A | Yes | ||
Enzyme | Proteinase K | N/A | Proteinase K | N/A | Lysozyme Proteinase K | Lyticase or Zymolase | Proteinase K | Proteinase K | ||
Typical Yield | 5 µg from 200 µl blood | 4-10 µg from 300 µl blood | 10-40 µg from 20 mg tissue | 3-5 µg from 100 mg Arabidopsis leaf (Mini) | 37 µg from 1x109 E. coli | 10-15 µg from 2x108 S. cerevisiae cells | 300 ng from 6 mm blood spots | 2 µg per swab | ||
10-40 µg from 20 mg tissue | 80 µg from 500 mg Lemon leaf (Maxi) | 10-15 µg from 1x109 Bacillus subtilis | ||||||||
Elution Volume | 30-100 µl | 30-200 µl | 30-200 µl | 30-200 µl (Mini) 1 ml (Maxi) | 30-200 µl | 50-100 µl | 30-100 µl | 50-100 µl | ||
Operation Time | <20 minutes | <25 minutes | 30 minutes | <30 minutes | <30 minutes | <40 minutes | <20 minutes | <20 minutes |
Product Name | RXNS | Catalogue Number | ||
gSYNC™ DNA Extraction Kit (Tissue/Blood) | 100, 300 | GS100/300 | ||
Genomic DNA Mini Kit (Blood/Cultured Cell) | 100, 300 | GB100/300 | ||
Genomic DNA Maxi Kit (Plant) | 10, 25 | GPM010/25 | ||
Genomic DNA Maxi Kit (Blood/Cultured Cell) | 10, 25 | GDM10/25 | ||
Genomic DNA Mini Kit (Tissue) | 50, 100, 300 | GT050/100/300 | ||
Genomic DNA Mini Kit (Plant) | 100 | GP100 | ||
Geneius™ Micro gDNA Extraction Kit | 100 | GMB100/300 | ||
Presto™ Buccal Swab gDNA Extraction Kit | 100 | GSK100/300 | ||
Presto™ Mini gDNA Yeast Kit | 100, 300 | GBYB100/300 | ||
Presto™ Mini gDNA Bacteria Kit | 100, 300 | GBB100/101/300/301 | ||
Presto™ 96 Well Blood Genomic DNA Extraction Kit | 4 x 96, 10 x 96 | 96GBP04/10 | ||
Presto™ 96 Well Plant Genomic DNA Extraction Kit | 4 x 96, 10 x 96 | 96GPP04/10 | ||
Presto™ 96 Well gDNA Bacteria Kit | 4 x 96, 10 x 96 | 96GBB04/10 |