Gel/PCR DNA Fragments Extraction Kit

DNA Extraction/Gel Extraction

The Gel/PCR DNA Fragments Extraction Kit is a Gel Extraction Kit and PCR Cleanup Kit in 1 convenient system. This Gel Extraction Kit will recover or concentrate 70 bp-20 kb DNA fragments from agarose gel, PCR, or other enzymatic reactions. For processing 10-50 kb DNA fragments, the Large DNA Fragments Extraction Kit is available. For processing 40-200 bp DNA fragments, the Small DNA Fragments Extraction Kit is also available. The Gel/PCR DNA Fragments Extraction Kit uses chaotropic salt to dissolve agarose gel and denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the gel/pcr spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation and the purified DNA is ready for use in subsequent reactions such as Fluorescent and Radioactive Sequencing, PCR, Restriction Enzyme Digestion, DNA Labeling and Ligation, Microarray and Transfection.

Specifications (Cat. # DF100, DF300)

High Efficiency: up to 90% recovery from agarose gels and up to 95% recovery from PCR products or other enzymatic reactions
Convenient: gel extraction kit and PCR cleanup kit in one
Only 20 minute gel extraction or 10 minute PCR cleanup
Sample Size: up to 300 mg of agarose gel slice or up to 100 μl of PCR products
Broad Fragment Size Range: 70 bp–20 kb
Spin Columns: glass fiber membrane optimized for both gel extraction and PCR cleanup
Elution Volume: 20-50 µl
Storage: dry at room temperature (15-25ºC)

Fluorescent and Radioactive Sequencing,
PCR,
Restriction Enzyme Digestion,
DNA Labeling and Ligation,
Microarray,
Transfection

Components

DF Buffer
W1 Buffer
Wash Bufffer
Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
DF Columns
2 ml Collection Tubes

Quality Control

The quality of the Gel/PCR DNA Fragments Extraction Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. DNA fragments are purified from either agarose gel, PCR products or other aqueous solutions and analyzed by electrophoresis.

Gel Extraction & PCR Cleanup Kits Specifications

Specifications	GenepHlow™ Gel Extraction Kit	GenepHlow™ PCR Cleanup Kit	GenepHlow™ Gel/PCR Kit	GenepHlow™ DNA Cleanup Maxi Kit	Presto™ 96 Well PCR Cleanup Kit	Small DNA Fragments Extraction Kit	Presto™ Max Gel/PCR Kit
Cat. #	DFG100 DFG300	DFC100 DFC300	DFH100 DFH300	DFM010 DFM025	96DFH04 96DFH10	DF101 DF301	DFL100 DFL300
Format	Spin Column	Spin Column	Spin Column	Maxi Spin Column	Spin Column	Spin Column	Silica Matrix
Binding Capacity	10 µg	10 µg	10 µg	200 µg	10 µg	10 µg	5 µg
Sample	Agarose Gel Slice	PCR Product	Agarose Gel Slice/PCR Product	Agarose Gel Slice/Restriction Enzyme Digest Product	PCR Product	Agarose Gel Slice/PCR Product	Agarose Gel Slice/PCR Product
Fragment Size	70 bp-20 kb	70 bp-20 kb	70 bp-20 kb	100 bp-10 kb	70 bp-20 kb	40 bp-200 bp	100 bp-50 kb
pH Indicator	Yes	Yes	Yes	Yes	N/A	N/A	Yes
Recovery	Up to 90%	Up to 95%	Up to 95%	Up to 80%	Up to 95%	Up to 95%	Up to 95% (<10 kb) Up to 80% (10-50 kb)
Elution Volume	20-50 µl	20-50 µl	20-50 µl	500-1000 µl	60-80 µl	20-50 µl	10-20 µl
Operation Time	<20 minutes	<10 minutes	<20 minutes	<25 minutes	<20 minutes	<20 minutes	<20 minutes

Gel:PCR DNA Fragments Extraction Kit

Product Name	RXNS	Catalogue Number
GenepHlow™ Gel Extraction Kit	100/300	DFG100/300
GenepHlow™ PCR Cleanup Kit	100/300	DFC100/300
GenepHlow™ Gel/PCR Kit	100/300	DFH100/300
GenepHlow™ DNA Cleanup Maxi Kit	10/25	DFM010/25
Presto™ 96 Well PCR Cleanup Kit	4 x 96/10 x 96	96DFH04/10
Small DNA Fragments Extraction Kit	100/300	DF101/301
Presto™ Max Gel/PCR Kit	100/300	DFL100/300
Gel/PCR DNA Fragments Extraction Kit	100/300	DF100/300
Presto™ 96 Well Gel Extraction Kit	4 x 96/10 x 96	96DFG04/10