Geneaid™ DNA Isolation Kit (Yeast)
Reagent DNA Extraction/Reagent DNA Extraction
The Geneaid™ DNA Isolation Kit (Yeast) offers a simple and gentle reagent DNA precipitation method for isolating protein free, high molecular weight genomic, mitochondrial or viral DNA from Saccharomyces cerevisiae and a variety of other yeast and fungus species. This highly versatile solution based system offers a convenient procedure with minimal hands on time. The provided Sorbitol Buffer, when combined with zymolase or lyticase, will efficiently lyse yeast and other fungus species cell walls consisting of chitin and polysaccharides. Purified DNA (A260/A280 = 1.8-2.0), is suitable for use in PCR or other enzymatic reactions.
Specifications (Cat. # GEY100, GEY300)
- Purify genomic DNA from (up to 2 x 108) yeast and other fungus species using a simple and gentle 3 buffer system
- Simple and gentle reagent DNA precipitation method
- Convenient premixed Sorbitol Buffer!
- Time: 40 minutes
- Elution volume: 50-100 µl
- Storage: dry at room temperature (15-25°C)
- DNA archiving,
- PCR,
- AFLP,
- RFLP/PADP,
- Southern Blotting,
- Real-time PCR
Components
- Sorbitol Buffer
- Cell Lysis Buffer
- Protein Removal Buffer
- DNA Hydration Buffer
Quality Control
The quality of the Presto™ Mini gDNA Yeast Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. The purified genomic DNA (A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.
Reagent Genomic DNA Extraction Kits Specifications
| Specifications | Geneaid™ DNA Isolation Kit Blood | Geneaid™ DNA Isolation Kit Cultured Cell | Geneaid™ DNA Isolation Kit Tissue | Geneaid™ DNA Isolation Kit Bacteria | Geneaid™ DNA Isolation Kit Yeast | GENEzol™ DNA Reagent Plant | ||
| Cat. # | GEB100 GEB01K GEB01K+ | GEC150 GEC1.5K GEC1.5K+ | GET150 GET1.5K GET1.5K+ | GEE150 GEE1.5K GEE1.5K+ | GEY100 GEY300 | GR100 GR200 | ||
| RXNS | 100-1,000 | 150-1,500 | 150-1,500 | 150-1,500 | 100-300 | 100-200 | ||
| Format | Reagent System | Reagent System | Reagent System | Reagent System | Reagent System | Reagent System | ||
| Sample | 300 µl-10 ml blood | up to 5x107 cultured cells | up to 200 mg tissue | up to 1.5x109 bacterial cells | up to 2x108 yeast cells | up to 1 g plant tissue | ||
| Elution Volume | 100 µl-1 ml | 50-200 µl | 100-200 µl | 100 µl | 50-100 µl | 50-100 µl | ||
| Operation Time | <40 minutes | <60 minutes | <70 minutes | <60 minutes | <40 minutes | <50 minutes |
| Tablo 1 | ||||
| Product Name | RXNS | Catalogue Number | ||
| Geneaid™ DNA Isolation Kit (Bacteria) | 150/1,500 | GEE100/01K/01K+ | ||
| Geneaid™ DNA Isolation Kit (Blood) | 100/1,000 | GEB100/01K/01K+ | ||
| Geneaid™ DNA Isolation Kit (Tissue) | 150/1,500 | GET150/1.5K/1.5K+ | ||
| Geneaid™ DNA Isolation Kit (Cultured Cell) | 150/1,500 | GEC150/1.5K/1.5K+ | ||
| Geneaid™ DNA Isolation Kit (Yeast) | 100/300 | GEY100/300 | ||
| GENEzol™ DNA Reagent Plant | 100/200 | GR100/200 | ||
