GenepHlow™ DNA Cleanup Midi Kit
DNA Extraction/PCR Cleanup
GenepHlow™ DNA Cleanup Midi Kits were designed to recover or concentrate DNA fragments from PCR or other enzymatic reactions. This DNA cleanup kit includes a pH indicator as an optional addition to the PB binding buffer to ensure optimal pH and facilitate DNA binding. If pH exceeds the optimal level (>7.5), the color of the solution will appear purple instead of yellow. 3M Sodium Acetate (pH5.0) which is included with the kit, can then be added to the solution to adjust pH and return the color to yellow. Chaotropic salt is used to denature enzymes while DNA fragments are bound by the glass fiber matrix of the spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. The pH indicator, salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation and the purified DNA is ready for use in subsequent reactions.
Specifications (Cat. # DFI100, DFI300)
- High Efficiency: up to 90% recovery
- Convenient: includes pH indicator for easy determination of optimal pH and sodium acetate to adjust pH if it becomes too high
- Includes 3M Sodium Acetate (pH5.0) to adjust pH if it becomes too high
- Sample Size: 10-40 µg of DNA in up to 200 µl PCR/Digestion solutions
- Broad Fragment Size Range: 100 bp–20 kb
- Operation Time: 10 minutes
- Spin Columns: glass fiber membrane
- Elution Volume: 30-50 µl
- Storage: dry at room temperature (15-25°C)
- Fluorescent and Radioactive Sequencing,
- PCR,
- Restriction Enzyme Digestion,
- DNA Labeling and Ligation,
- Microarray,
- Transfection
Components
- PB Buffer
- pH Indicator
- 3M Sodium Acetate (pH5.0)
- Wash Bufffer
- Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
- DF Midi Columns
- 2 ml Collection Tubes
Quality Control
The quality of the GenepHlow™ DNA Cleanup Midi Kit is tested on a lot-to-lot basis by purifying DNA fragments of various sizes from PCR products, digestion products or other aqeous solutions. The purified DNA is analyzed by electrophoresis.
Gel Extraction & PCR Cleanup Kits Specifications
| Specifications | GenepHlow™ Gel Extraction Kit | GenepHlow™ PCR Cleanup Kit | GenepHlow™ Gel/PCR Kit | GenepHlow™ DNA Cleanup Maxi Kit | Presto™ 96 Well PCR Cleanup Kit | Small DNA Fragments Extraction Kit | Presto™ Max Gel/PCR Kit | ||
| Cat. # | DFG100 DFG300 | DFC100 DFC300 | DFH100 DFH300 | DFM010 DFM025 | 96DFH04 96DFH10 | DF101 DF301 | DFL100 DFL300 | ||
| Format | Spin Column | Spin Column | Spin Column | Maxi Spin Column | Spin Column | Spin Column | Silica Matrix | ||
| Binding Capacity | 10 µg | 10 µg | 10 µg | 200 µg | 10 µg | 10 µg | 5 µg | ||
| Sample | Agarose Gel Slice | PCR Product | Agarose Gel Slice/PCR Product | Agarose Gel Slice/Restriction Enzyme Digest Product | PCR Product | Agarose Gel Slice/PCR Product | Agarose Gel Slice/PCR Product | ||
| Fragment Size | 70 bp-20 kb | 70 bp-20 kb | 70 bp-20 kb | 100 bp-10 kb | 70 bp-20 kb | 40 bp-200 bp | 100 bp-50 kb | ||
| pH Indicator | Yes | Yes | Yes | Yes | N/A | N/A | Yes | ||
| Recovery | Up to 90% | Up to 95% | Up to 95% | Up to 80% | Up to 95% | Up to 95% | Up to 95% (<10 kb) Up to 80% (10-50 kb) | ||
| Elution Volume | 20-50 µl | 20-50 µl | 20-50 µl | 500-1000 µl | 60-80 µl | 20-50 µl | 10-20 µl | ||
| Operation Time | <20 minutes | <10 minutes | <20 minutes | <25 minutes | <20 minutes | <20 minutes | <20 minutes |
| Product Name | RXNS | Catalogue Number | ||
| GenepHlow™ Gel Extraction Kit | 100/300 | DFG100/300 | ||
| GenepHlow™ PCR Cleanup Kit | 100/300 | DFC100/300 | ||
| GenepHlow™ Gel/PCR Kit | 100/300 | DFH100/300 | ||
| GenepHlow™ DNA Cleanup Maxi Kit | 10/25 | DFM010/25 | ||
| Presto™ 96 Well PCR Cleanup Kit | 4 x 96/10 x 96 | 96DFH04/10 | ||
| Small DNA Fragments Extraction Kit | 100/300 | DF101/301 | ||
| Presto™ Max Gel/PCR Kit | 100/300 | DFL100/300 | ||
| Gel/PCR DNA Fragments Extraction Kit | 100/300 | DF100/300 | ||
| Presto™ 96 Well Gel Extraction Kit | 4 x 96/10 x 96 | 96DFG04/10 |
