GenepHlow™ Gel Extraction Kit
DNA Extraction/Gel Extraction
The GenepHlow™ Gel Extraction Kit was designed to recover or concentrate DNA fragments from agarose gel. This gel extraction kit includes QG Buffer premixed with a pH indicator to ensure optimal pH, facilitate DNA binding and allow for easy observation of undissolved agarose gel. If pH exceeds the optimal level (>7.5), the color of the solution will appear purple instead of yellow. 3M Sodium Acetate (pH5.0), which is included with the kit, can then be added to the solution to adjust pH and return the color to yellow. Chaotropic salt is used to dissolve agarose gel and denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. The pH indicator, salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation and the purified DNA is ready for use in subsequent reactions.
Specifications (Cat. # DFG100, DFG300)
- High Efficiency: up to 90% recovery from agarose gels
- Only 20 minutes gel extraction
- Convenient: QG binding buffer is premixed with a pH indicator to allow for easy determination of optimal pH
- Includes 3M Sodium Acetate (pH5.0) to adjust pH if it becomes too high following gel dissociation
- Sample Size: up to 300 mg of agarose gel slice
- Broad Fragment Size Range: 70 bp–20 kb
- Spin Columns: glass fiber membrane optimized for gel extraction
- Elution Volume: 20-50 µl
- Storage: dry at room temperature (15-25°C)
- Fluorescent and Radioactive Sequencing,
- PCR,
- Restriction Enzyme Digestion,
- DNA Labeling and Ligation,
- Microarray,
- Transfection
Components
- QG Buffer (premixed with a pH indicator)
- 3M Sodium Acetate (pH5.0)
- W1 Buffer
- Wash Bufffer
- Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
- DFH Columns
- 2 ml Collection Tubes
Quality Control
The quality of the GenepHlow™ Gel Extraction Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. DNA fragments are purified from agarose gel and analyzed by electrophoresis.
Gel Extraction & PCR Cleanup Kits Specifications
| Specifications | GenepHlow™ Gel Extraction Kit | GenepHlow™ PCR Cleanup Kit | GenepHlow™ Gel/PCR Kit | GenepHlow™ DNA Cleanup Maxi Kit | Presto™ 96 Well PCR Cleanup Kit | Small DNA Fragments Extraction Kit | Presto™ Max Gel/PCR Kit | ||
| Cat. # | DFG100 DFG300 | DFC100 DFC300 | DFH100 DFH300 | DFM010 DFM025 | 96DFH04 96DFH10 | DF101 DF301 | DFL100 DFL300 | ||
| Format | Spin Column | Spin Column | Spin Column | Maxi Spin Column | Spin Column | Spin Column | Silica Matrix | ||
| Binding Capacity | 10 µg | 10 µg | 10 µg | 200 µg | 10 µg | 10 µg | 5 µg | ||
| Sample | Agarose Gel Slice | PCR Product | Agarose Gel Slice/PCR Product | Agarose Gel Slice/Restriction Enzyme Digest Product | PCR Product | Agarose Gel Slice/PCR Product | Agarose Gel Slice/PCR Product | ||
| Fragment Size | 70 bp-20 kb | 70 bp-20 kb | 70 bp-20 kb | 100 bp-10 kb | 70 bp-20 kb | 40 bp-200 bp | 100 bp-50 kb | ||
| pH Indicator | Yes | Yes | Yes | Yes | N/A | N/A | Yes | ||
| Recovery | Up to 90% | Up to 95% | Up to 95% | Up to 80% | Up to 95% | Up to 95% | Up to 95% (<10 kb) Up to 80% (10-50 kb) | ||
| Elution Volume | 20-50 µl | 20-50 µl | 20-50 µl | 500-1000 µl | 60-80 µl | 20-50 µl | 10-20 µl | ||
| Operation Time | <20 minutes | <10 minutes | <20 minutes | <25 minutes | <20 minutes | <20 minutes | <20 minutes |
| Product Name | RXNS | Catalogue Number | ||
| GenepHlow™ Gel Extraction Kit | 100/300 | DFG100/300 | ||
| GenepHlow™ PCR Cleanup Kit | 100/300 | DFC100/300 | ||
| GenepHlow™ Gel/PCR Kit | 100/300 | DFH100/300 | ||
| GenepHlow™ DNA Cleanup Maxi Kit | 10/25 | DFM010/25 | ||
| Presto™ 96 Well PCR Cleanup Kit | 4 x 96/10 x 96 | 96DFH04/10 | ||
| Small DNA Fragments Extraction Kit | 100/300 | DF101/301 | ||
| Presto™ Max Gel/PCR Kit | 100/300 | DFL100/300 | ||
| Gel/PCR DNA Fragments Extraction Kit | 100/300 | DF100/300 | ||
| Presto™ 96 Well Gel Extraction Kit | 4 x 96/10 x 96 | 96DFG04/10 |
