Magnetic Beads Genomic DNA Extraction Kit (Bacteria)
Magnetic Beads DNA/RNA Extraction/Magnetic Beads gDNA kit Bacteria
The Magnetic Beads Genomic DNA Extraction Kit Bacteria was designed specifically for efficient genomic DNA purification from Gram (-) negative and Gram (+) positve bacteria. DNA is bound to the surface of the magnetic beads and released using a proprietary buffer system. The kit can be easily adapted to automated magnetic bead separation instruments and workstations. The purified DNA can be used in qPCR and a variety of other downstream applications.
Advantages (Cat. # ME048, ME096)
- High Yield: >26 µg of Genomic DNA
- High Quality DNA: A260/A280 = 1.8-2.0
- Magnetic Bead Concentration: 50 mg/ml
- Magnetic Bead Size: ~ 5 µm
- Easily adapted to automated magnetic bead separation instruments and workstations
- Sample: 2 x 109 of bacteria cells (Gram + and Gram -)
- Manual or automated DNA isolation
- Operation time: within 30 minutes (manual)
- Storage: dry at room temperature (15-25ºC), Lysozyme is shipped at room temperature and should be stored at -20°C for extended periods
- qPCR,
- PCR,
- Real-time PCR,
- Automated Fluorescent DNA Sequencing,
- Southern Blotting,
- Next Generation Sequencing (NGS)
Components
- ME1 Buffer
- ME2 Buffer
- ME3 Buffer
- ME4 Buffer
- Lysozyme
- Proteinase K
- MW1 Buffer
- MW2 Buffer
- ME Magnetic Beads
- Elution Buffer
Quality Control
The quality of the The Magnetic Beads Genomic DNA Extraction Kit Bacteria is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system by isolating genomic DNA from 1 x 109 cells of Escherichia coli and from 1 x 109 cells of Bacillus subtilis. 5 μl of extracted DNA product from a 100 μl eluate is analyzed on a 0.8% agarose gel.
| Product Name | Rxns/Volume | Catalogue # |
|---|---|---|
| Magnetic Beads Genomic DNA Extraction Kit (Blood) | 48/96 | MB048/96 |
| Magnetic Beads Viral DNA/RNA Extraction Kit | 48/96 | MV048/96 |
| Magnetic Beads Genomic DNA Extraction Kit (Bacteria) | 48/96 | ME048/96 |
| Magnetic Beads PCR Cleanup Kit | 48/96 | MC048/96 |
| Magnetic Beads Micro gDNA Extraction Kit | 48/96 | MM048/96 |
| Magnetic Beads gSYNC DNA Kit (Tissue) | 48/96 | MGS048/96 |
| Magnetic Beads Soil Genomic DNA Extraction Kit | 48/96 | MS048/96 |
