Presto™ 96 Well Plant Genomic DNA Extraction Kit
DNA Extraction/Genomic DNA Purification
The Presto™ 96 Well Plant Genomic DNA Extraction Kit is designed for high-throughput purification of total DNA (including genomic DNA, mitochondrial and chloroplast DNA) from various plant species. Homogenized samples are treated with RNase A then centrifuged to remove cell debris and salt precipitates. In the presence of a binding buffer, genomic DNA in the lysate binds to the glass fiber matrix of the Presto™ 96 Well gDNA Binding Plate. Contaminants are removed using a Wash Buffer and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed within 60 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA (approximately 20-30 kb) is suitable for use in PCR or other enzymatic reactions.
Specifications (Cat. # 96GPP04, 96GPP10)
- Purify 96 wells of pure genomic DNA from a variety of plant species in 60 minutes!
- Sample Volume: 100 mg of fresh plant tissue and 25 mg of dried plant tissue per well
- Yield: up to 30 μg of genomic DNA from 100 mg of fresh plant tissue per well
- Format: Presto™ 96 Well gDNA Binding Plates
- Elution Volume: 200~400 µl
- Centrifuge or vacuum manifold protocol
- Storage: dry at room temperature (15-25ºC)
- PCR,
- AFLP/PADP,
- RFLP,
- Southern Blotting,
- Real-time PCR
Components
- GP1 Buffer and GPX1 Buffer
- GP2 Buffer
- GP3 Buffer
- RNase A
- W1 Buffer
- Wash Buffer
- Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
- Presto™ gDNA 96 Well Binding Plates
- 96 Deep Well Plates
- Microtubes (Racked)
- Microtubes (8-strip)
- Caps for Microtubes (8-strip)
Quality Control
The quality of the Presto™ 96 Well Plant Genomic DNA Extraction Kit is tested on a lot-to-lot basis by purifying genomic DNA from corn leaf samples. The purified DNA is quantified with a spectrophotometer and analyzed by electrophoresis.
| Product Name | RXNS | Catalogue Number | ||
| gSYNC™ DNA Extraction Kit (Tissue/Blood) | 100, 300 | GS100/300 | ||
| Genomic DNA Mini Kit (Blood/Cultured Cell) | 100, 300 | GB100/300 | ||
| Genomic DNA Maxi Kit (Plant) | 10, 25 | GPM010/25 | ||
| Genomic DNA Maxi Kit (Blood/Cultured Cell) | 10, 25 | GDM10/25 | ||
| Genomic DNA Mini Kit (Tissue) | 50, 100, 300 | GT050/100/300 | ||
| Genomic DNA Mini Kit (Plant) | 100 | GP100 | ||
| Geneius™ Micro gDNA Extraction Kit | 100 | GMB100/300 | ||
| Presto™ Buccal Swab gDNA Extraction Kit | 100 | GSK100/300 | ||
| Presto™ Mini gDNA Yeast Kit | 100, 300 | GBYB100/300 | ||
| Presto™ Mini gDNA Bacteria Kit | 100, 300 | GBB100/101/300/301 | ||
| Presto™ 96 Well Blood Genomic DNA Extraction Kit | 4 x 96, 10 x 96 | 96GBP04/10 | ||
| Presto™ 96 Well Plant Genomic DNA Extraction Kit | 4 x 96, 10 x 96 | 96GPP04/10 | ||
| Presto™ 96 Well gDNA Bacteria Kit | 4 x 96, 10 x 96 | 96GBB04/10 |
