Presto™ cfDNA/RNA Extraction Kit II
DNA/RNA/Protein Extraction/Presto™ cfDNA/RNA Extraction Kit
The Presto™ cfDNA/RNA Extraction Kit II is designed for rapid isolation of high-quality circulating cell-free DNA/RNA from up to 4 ml of serum and plasma. The biological liquid sample is lysed with the Lysis Buffer containing chaotropic salts. The proteins in the sample lysate are then palleted with Precipitation Buffer and the cfDNA/RNA is bound with the CF column. The unique designed Column Extender allows the extraction procedure can be performed either using a vacuum manifold or a centrifuge with the swing bucket. The CF column is washed with Wash Buffer and cfDNA/RNA is eluted with RNase-free Water. The entire procedure can be completed within 40 minutes and the purified cfDNA/RNA is ready for use in all subsequent analyses and molecular manipulations such as qPCR, Next-Generation sequencing and DNA methylation analyses.
Advantages (Cat. # CF025, CF050, CF100)
- Samples: 1-4 ml of serum or plasma
- Yield: 1-100 ng /ml serum or plasma
- Includes column extension tubes for increased sample volume and yield
- Elution Volume: 30-50 μl
- Storage: dry at room temperature (15-25°C)
- qPCR,
- Next-Generation sequencing,
- DNA methylation analysis
Components
- CFL Buffer
- CFP Buffer
- W1 Buffer
- Wash Buffer
- RNase-free Water
- Carrier RNA
- Column Extension Tubes
- CF Column
- 2 ml Collection Tubes
Quality Control
The quality of the Presto™ cfDNA/RNA Extraction Kit II is tested on a lot-to-lot basis by isolating cfDNA/RNA from 1 ml of plasma. Following the purification process, the purified cfDNA/RNA integrity is assessed by qPCR.
