Presto™ DNA RNA Protein Extraction Kit
DNA/RNA/Protein Extraction/Presto™ DNA/RNA/Protein Extraction Kit
The Presto™ DNA RNA Protein Extraction Kit provides an efficient method for purifying genomic DNA, total RNA and total protein simultaneously from cultured cells, animal tissue, whole blood and biological fluids such as serum and plasma. Chaotropic salt is used to lyse cells and degrade protein, allowing DNA to bind to the genomic DNA mini spin column. The flow-through containing RNA can then be transferred to the RNA mini spin column for RNA binding. The proteins in the flow-through can then be precipitated using acetone. Contaminants are removed using a wash buffer and the purified genomic DNA is eluted in a low salt Elution Buffer and the purified total RNA is eluted in RNase-free Water. The entire procedure can be completed in 25 minutes without phenol/chloroform extraction or alcohol precipitation. The purified DNA, approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions and the purified RNA (including miRNA) is ready for use in RT-PCR, Real-time PCR, northern blotting, primer extension, mRNA selection and cDNA synthesis. The purified proteins can be directly analyzed on a SDS-PAGE and subsequent western blot.
Advantages (Cat. # DRP050, DRP100)
- Purify genomic DNA, total RNA within 25 minutes! Protein precipitation within 50 minutes!
- Variety of Samples: up to 500 μl of whole blood, up to 25 mg of tissue, rodent tails, ear punches, up to 5 x 106 cultured cells, up to 200 μl of biological fluids such as serum and plasma
- High Yield: up to 9 μg of genomic DNA, up to 20 μg of total RNA from 1.5 x 106 of cultured HeLa cells, up to 1 μg of genomic DNA, up to 10 μg of total RNA from 500 µl human blood samples
- High Quality DNA: A260/A280 = 1.9-2.0
- Genomic DNA glass fiber spin columns and total RNA glass fiber spin colums certified DNase and RNase-free
- Elution Volume: 50-200 μl (genomic DNA) and 25-50 μl (total RNA)
- Storage: dry at room temperature (15-25°C)
The purified DNA, approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions and the purified RNA (including miRNA) is ready for use in RT-PCR, Real-time PCR, northern blotting, primer extension, mRNA selection and cDNA synthesis. The purified proteins can be directly analyzed on a SDS-PAGE and subsequent western blot.
Components
- RBC Lysis Buffer
- DR Buffer
- RW1 Buffer
- RPE Buffer
- W1 Buffer
- Wash Buffer
- RNase-free Water
- Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
- DV Buffer (8M Urea)
- Protein Loading Dye
- GD Columns
- RB Columns
- 2 ml Collection Tubes
Quality Control
The quality of the Presto™ DNA/RNA/Protein Extraction Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system by isolating genomic DNA, total RNA and total protein from 1.5 x 106 cultured animal cells. The purified DNA and total RNA is quantified with a spectrophotometer and analyzed by electrophoresis on a 1% agarose gel. The purified protein is quantified by bradford assay and analyzed on SDS-PAGE.
| Product Name | RXNS | Catalogue # |
|---|---|---|
| Presto™ DNA/RNA/Protein Extraction Kit | 50/100 | DRP050/100 |
| GENEzol™ Reagent | 50/100/200 | GZR050/100/200 |
| GENEzol™ TriRNA Bacteria Kit | 50/100 | GZB050/100 |
| Viral Nucleic Acid Extraction Kit II | 50/100/300 | VR050/100/300 |
| Viral Nucleic Acid Extraction Kit III | 50/100/300 | VI050/100/300 |
