Presto™ FFPE RNA Mini Kit with DNase
RNA Extraction/Total RNA
The Presto™ FFPE RNA Mini Kit was designed specifically for purifying total RNA from formalin-fixed, paraffin-embedded (FFPE) tissue. Paraffin is initially dissolved with organic solvents such as Xylene followed by Proteinase K digestion. RNA in chaotropic salt is then bound by the glass fiber matrix of the spin column and DNA contamination is removed using an in column DNase treatment. Other contaminants are removed using Wash Buffer and the purified total RNA is eluted in RNase-free Water. High quality total RNA can be purified within 80 minutes without phenol extraction or alcohol precipitation. The purified RNA is ready for use in Real-time PCR, mRNA selection, cDNA synthesis and NGS.
Specifications (Cat. # RF050, RF100)
- Sample: up to 25 mg sections of FFPE, 4~10 sections of 5~20 μm thick FFPE
- High Yield: up to 50 μg of total RNA
- Spin Columns: glass fiber membrane optimized for total RNA extraction
- Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
- Storage: Storage: dry at room temperature (15-25°C)
- Operation Time: within 80 minutes
- Real-time PCR,
- mRNA Selection,
- cDNA Synthesis,
- NGS
Components
- RT Buffer
- RB Buffer
- Proteinase K
- DNase I
- DNase I Reaction Buffer
- W1 Buffer
- Wash Buffer
- RNase-free Water
- RB Columns
- 2 ml Collection Tubes
Quality Control
The quality of the Presto™ FFPE RNA Mini Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. Total RNA is isolated from 25 mg sections of FFPE, quantified with a spectrophotometer and analyzed by electrophoresis.
Total RNA Extraction Kits Specifications
| Specifications | Total RNA Mini Kit Blood Cultured Cell | Total RNA Mini Kit Tissue | Total RNA Mini Kit Plant | Presto™ Mini RNA Bacteria Kit | Presto™ Mini RNA Yeast Kit | miRNA Isolation Kit | GENEzol™ TriRNA Pure Kit | ||
| Cat. # | RB050 RB100 RB300 | RT050 RT100 RT300 | RP050 RP100 RP300 | RBB050 RBB100 RBB300 | RBY050 RBY100 RBY300 | RMI050 | GZX050 GZX100 GZX200 | ||
| Format | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Tri Reagent Spin Column | ||
| Binding Capacity | 50 µg | 50 µg | 50 µg | 50 µg | 50 µg | 50 µg | 50 µg | ||
| Sample | blood, cells | animal tissue | plant tissue | bacterial cells | yeast cells | animal tissue, cells | blood,cells, tissue, body fluids, bacteria | ||
| Micropestle | N/A | Yes | N/A | N/A | N/A | Yes | N/A | ||
| Filter Column | N/A | Yes | Yes | N/A | N/A | N/A | N/A | ||
| Enzyme | N/A | N/A | N/A | Lysozyme | N/A | N/A | N/A | ||
| Typical Yield | 2-3 µg from 300 µl blood, 25-30 µg from 106 cells | 5-30 µg | 5-30 µg | 40-50 µg from 1x109 bacterial cells | 15-20 µg from 5x107 yeast cells | 5-30 µg | 5-30 µg | ||
| Elution Volume | 50 µl | 50 µl | 50 µl | 50 µl | 50 µl | 50 µl | 25-50 µl | ||
| Time | <20 min. | <15 min. | <20 min. | <20 min. | <20 min. | <30 min. | <15 min. |
| Product Name | RXNS | Catalogue Number | ||
| Total RNA Mini Kit (Blood/Cultured Cell) | 50/100/300 | RB050/100/300, RBD050/100/300 | ||
| Total RNA Mini Kit (Tissue) | 50/100/300 | RT050/100/300, RTD050/100/300 | ||
| Total RNA Mini Kit (Plant) | 50/100/300 | RP050/100/300, RPD050/100/300 | ||
| Presto™ Mini RNA Bacteria Kit | 50/100/300 | RBB050/100/300, RBBD050/100/300 | ||
| Presto™ Mini RNA Yeast Kit | 50/100/300 | RBY050/100/300, RBYD050/100/300 | ||
| miRNA Isolation Kit | 50 | RMI050 | ||
| GENEzol™ TriRNA Pure Kit | 50/100/200 | GZX050/100/200, GZXD050/100/200 | ||
| GENEzol™ 96 Well TriRNA Pure Kit | 4 x 96/10 x 96 | 96GZX04/10 |
