{"id":14275,"date":"2024-03-12T15:00:43","date_gmt":"2024-03-12T15:00:43","guid":{"rendered":"https:\/\/sightgen.com\/?post_type=product&#038;p=14275"},"modified":"2024-03-13T10:18:17","modified_gmt":"2024-03-13T10:18:17","slug":"midi-spin-column-plasmid-kit","status":"publish","type":"product","link":"https:\/\/sightgen.com\/en\/product\/midi-spin-column-plasmid-kit\/","title":{"rendered":"Midi Spin Column Plasmid Kit"},"content":{"rendered":"","protected":false},"excerpt":{"rendered":"<h3>DNA Extraction\/Plasmid DNA Purification<\/h3>\n<div class=\"txt\">\n<p>The Midiprep Spin Column Plasmid Kit was designed for plasmid and cosmid DNA purification from 50-100 ml of cultured bacterial cells using a plasmid midiprep spin column system. This plasmid kit uses a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA\/RNA contaminants. In the presence of chaotropic salt, plasmid DNA in the lysate binds to the glass fiber matrix of the plasmid spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified plasmid DNA is eluted by a low salt Elution Buffer, TE or water. Typical yields are 200-350 \u03bcg for high-copy number plasmid or 30-100 \u03bcg for low-copy number plasmid from 50 ml of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 35 minutes.<\/p>\n<\/div>","protected":false},"featured_media":14276,"template":"","meta":[],"product_cat":[112],"product_tag":[],"_links":{"self":[{"href":"https:\/\/sightgen.com\/en\/wp-json\/wp\/v2\/product\/14275"}],"collection":[{"href":"https:\/\/sightgen.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sightgen.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sightgen.com\/en\/wp-json\/wp\/v2\/media\/14276"}],"wp:attachment":[{"href":"https:\/\/sightgen.com\/en\/wp-json\/wp\/v2\/media?parent=14275"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sightgen.com\/en\/wp-json\/wp\/v2\/product_cat?post=14275"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sightgen.com\/en\/wp-json\/wp\/v2\/product_tag?post=14275"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}