{"id":14324,"date":"2024-03-13T12:36:43","date_gmt":"2024-03-13T12:36:43","guid":{"rendered":"https:\/\/sightgen.com\/?post_type=product&#038;p=14324"},"modified":"2024-03-13T13:30:01","modified_gmt":"2024-03-13T13:30:01","slug":"high-speed-plasmid-mini-kit","status":"publish","type":"product","link":"https:\/\/sightgen.com\/en\/product\/high-speed-plasmid-mini-kit\/","title":{"rendered":"High-Speed Plasmid Mini Kit"},"content":{"rendered":"","protected":false},"excerpt":{"rendered":"<h4>DNA Extraction\/Plasmid DNA Purification<\/h4>\n<div class=\"txt\">\n<p>The High-Speed Plasmid Mini Kit was designed for both plasmid and cosmid DNA purification. This plasmid kit will purify plasmid DNA\/cosmid DNA from 1-5 ml of cultured bacterial cells using an efficient plasmid miniprep system. The High-Speed Plasmid Mini Kit uses a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA\/RNA contaminants. In the presence of chaotropic salt, plasmid DNA in the lysate binds to the glass fiber matrix of the plasmid spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified plasmid DNA is eluted by a low salt Elution Buffer, TE or water. Typical yields are 20-30 \u03bcg for high-copy number plasmid or 3-10 \u03bcg for low-copy number plasmid from 4 ml of cultured bacterial cells. This plasmid kit does not require DNA phenol extraction or alcohol precipitation and the entire procedure can be completed within 15 minutes.<\/p>\n<\/div>","protected":false},"featured_media":14329,"template":"","meta":[],"product_cat":[112],"product_tag":[],"_links":{"self":[{"href":"https:\/\/sightgen.com\/en\/wp-json\/wp\/v2\/product\/14324"}],"collection":[{"href":"https:\/\/sightgen.com\/en\/wp-json\/wp\/v2\/product"}],"about":[{"href":"https:\/\/sightgen.com\/en\/wp-json\/wp\/v2\/types\/product"}],"wp:featuredmedia":[{"embeddable":true,"href":"https:\/\/sightgen.com\/en\/wp-json\/wp\/v2\/media\/14329"}],"wp:attachment":[{"href":"https:\/\/sightgen.com\/en\/wp-json\/wp\/v2\/media?parent=14324"}],"wp:term":[{"taxonomy":"product_cat","embeddable":true,"href":"https:\/\/sightgen.com\/en\/wp-json\/wp\/v2\/product_cat?post=14324"},{"taxonomy":"product_tag","embeddable":true,"href":"https:\/\/sightgen.com\/en\/wp-json\/wp\/v2\/product_tag?post=14324"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}