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Presto™ 96 Well Plasmid Kit

DNA Extraction/Plasmid DNA Purification

The Presto™ 96 Well Plasmid Kit was designed for high-throughput purification of plasmid DNA from 1-5 ml of cultured bacterial cells per well using an efficient 96 well filter plate and 96 well binding plate system. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 16-20 μg for high-copy number plasmid or 3-10 μg for low-copy number plasmid from 4 ml of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 25 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.


  • Purify 96 wells of pure plasmid DNA in 25 minutes!
  • Sample Volume: 1-5 ml of cultured bacterial cells per well
  • Yield: 16-20 μg per well (high-copy plasmid), 3-10 μg per well (low-copy plasmid)
  • Binding Capacity: 35 μg per well
  • Plasmid Size Range: 1-20 kb
  • 96 Well plates for filtration and DNA binding
  • Elution Volume: 60 μl from 100 μl elution buffer volume
  • Centrifuge or vacuum manifold protocol
  • Storage: dry at room temperature (15-25ºC)

Quality Control

The quality of the Presto™ 96 Well Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 ml overnight E. coli (DH5α) culture containing plasmid pBluescript (A600 > 3.7 U/ml). Following the purification process, a yield of more than 16 μg per well is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.

Fluorescent and Radioactive Sequencing, Restriction Enzyme Digestion, Library Screening, Ligation, Transformation and PCR

Figure 1 .Sequencing data of (pBluescript) plasmid DNA purified using the Presto™ 96 Well Plasmid Kit.

E. coli Culture Volume260/280260/230Yield
1.5 ml 1.90 2.10 6-9 µg
4.0 ml 1.90 2.10 16-20 µg

Table 1 .Yield and quality of plasmid DNA purified using the Presto™ 96 Well Plasmid Kit. 5 µl aliquots of a 60 µl eluate of purified super coiled plasmid DNA from 1.5 ml and 4 ml overnight E. coli (DH5α) culture, containing a 3 kb plasmid pBluescript (OD600 = 3.7 U/ml) were EcoRI digestion and analyzed by electrophoresis on a 0.8% agarose gel.


  • P1, P2, P3 Buffers
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
  • RNase A
  • Presto™ Plasmid 96 Well Filter Plates
  • Presto™ Plasmid 96 Well Binding Plates
  • 96 Deep Well Plates
  • 0.35 ml Collection Plates
  • Adhesive Film
Specifications Presto™ Midi Plasmid Kit High-Speed Plasmid Mini Kit High-Speed Plasmid Mini Kit 10-50 Kb Presto™ 96 Well Plasmid Kit
Cat. # PDH100
Format Spin Column Spin Column Spin Column 96 Well Plate
Binding Capacity 50 µg 30 µg 30 µg 35 µg
Culture Input 1-7 ml 1-5 ml 1-4 ml 1-5 ml
Plasmid Size 1-15 kb 1-15 kb 10-50 kb 1-15 kb
Typical Yield up to 50 µg 10-30 µg 10-25 µg 20-35 µg/well
Elution Volume 30-100 µl 30-100 µl 50-100 µl 100 µl
Operation Time <15 minutes <15 minutes <15 minutes 25 minutes
Product NameRXNS/VOLUMECatalogue Number
Presto™ Endotoxin Free Mini Plasmid Kit100PEH100
Presto™ Mini Plasmid Kit100, 300PDH100/300
High-Speed Plasmid Mini Kit100, 300PD100/300
Large Plasmid DNA Extraction Kit100, 300PDL100/300
Midiprep Spin Column Plasmid Kit25PA025
Presto™ Midi Plasmid Kit25PIF025
Presto™ Midi Plasmid Kit (Endotoxin Free)25PIFE25
Geneaid™ Midi Plasmid Kit25PI025
Geneaid™ Midi Plasmid Kit (Endotoxin Free)25PIE25
Presto™ Plasmid DNA Concentration Kit25, 50, 100PC0250/500/1000
Geneaid™ Maxi Plasmid Kit10, 25PM010/25
Geneaid™ Maxi Plasmid Kit (Endotoxin Free)10, 25PME10/25
Presto™ 96 Well Plasmid Kit4 x 96, 10 x 9696PDC04/10