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EcoExtra™ Magnetic Beads Plasmid Kit

DNA Extraction/Plasmid DNA Purification

The EcoExtra™ Magnetic Beads Plasmid Kit was designed for high throughput purification of plasmid DNA from up to 16 ml of cultured bacterial cells per reaction using an efficient magnetic beads system. A modified alkaline lysis method and RNase treatment a re used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 6-10 μ g for high copy number plasmid from 1 ml of cultured E. coli cells. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions. More important, the EcoExtra™ series kits enable up to 80% reduction of plastic material waste compared to silica-column based kits. Make lab works a lot more eco friendly.

Specifications (Cat. # MPD192, MPD576, MPD1920)

  • Sample Volume: 1-16 ml of cultured bacterial cells
  • High Yield: 6-10 μg for high-copy number plasmid from 1 ml of cultured E. coli cells
  • High Purity plasmid DNA: A260/A280 =1.8-2.0, A260/230 =2.0-2.4
  • Plasmid DNA Size: 1-20 kb
  • Elution volume: 25-100 µl
  • Manual or automated plasmid DNA isolation
  • Reduce up to 80% of plastic material waste compared to spin-column based kits, more eco friendly!
  • Storage: dry at room temperature (15-25°C)
  • Restriction Enzyme Digestion,
  • Library Screening,
  • Ligation,
  • PCR,
  • Transformation and Sequencing Reactions


  • MPD1 Buffer
  • MPD2 Buffer
  • MPD3 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
  • RNase A
  • MPD Magnetic Beads

Quality Control

The quality of the EcoExtra™  Magnetic Beads Plasmid Kit  is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. Plasmid DNA from a 4 ml overnight E. coli (DH5α) culture, containing plasmid pBluescript is purified using the EcoExtra™  Magnetic Beads Plasmid Kit . Following the purification process, a yield of more than 30 μg is obtained and the A260/A280 ratio is between 1.8-2.0.

EcoExtra™  Magnetic Beads Plasmid Kit Functional Test Data




Figure 1 .Plasmid DNA was extracted using the EcoExtra™ Magnetic Beads Plasmid Kit. 5 µl aliquots of a 100 µl eluate of purified super coiled plasmid DNA from 1, 2, and 4 ml overnight E. coli (DH5α) culture, containing a 3 kb plasmid pBluescript (OD600 = 4 ). The purified plasmid were analyzed by electrophoresis on a 0.8% agarose gel.

Lane M = Geneaid 1 Kb DNA Ladder,  Lane1-2 =1 ml, Lane 3-4 =2 ml, Lane 5-6 =4 ml cultured E. coli cells



1ml E.coli cells6-10 µg1.8-2.02.0-2.4
2ml E.coli cells15-20 µg1.8-2.02.0-2.4
4ml E.coli cells26-32 µg1.8-2.02.0-2.4
8ml E.coli cells40-46 µg1.8-2.02.0-2.4
16ml E.coli cells70-78 µg1.8-2.02.0-2.4



Product NameRXNS/VOLUMECatalogue Number
Presto™ Endotoxin Free Mini Plasmid Kit100PEH100
Presto™ Mini Plasmid Kit100, 300PDH100/300
High-Speed Plasmid Mini Kit100, 300PD100/300
Large Plasmid DNA Extraction Kit100, 300PDL100/300
Midiprep Spin Column Plasmid Kit25PA025
Presto™ Midi Plasmid Kit25PIF025
Presto™ Midi Plasmid Kit (Endotoxin Free)25PIFE25
Geneaid™ Midi Plasmid Kit25PI025
Geneaid™ Midi Plasmid Kit (Endotoxin Free)25PIE25
Presto™ Plasmid DNA Concentration Kit25, 50, 100PC0250/500/1000
Geneaid™ Maxi Plasmid Kit10, 25PM010/25
Geneaid™ Maxi Plasmid Kit (Endotoxin Free)10, 25PME10/25
Presto™ 96 Well Plasmid Kit4 x 96, 10 x 9696PDC04/10