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Geneaid™ DNA Isolation Kit (Bacteria)

Reagent DNA Extraction/Reagent DNA Extraction

The Geneaid™ DNA Isolation Kit (Bacteria) offers a simple and gentle reagent DNA precipitation method for isolating protein free, high molecular weight genomic and viral DNA from Gram (+) positive and Gram (-) negative bacteria cells, suitable for archiving or sensitive downstream applications. This highly versatile solution based system can be scaled proportionately in order to satisfy larger sample volumes providing a convenient sample-storage procedure with minimal hands on time. Initially cells are lysed in the presence of detergents and a proprietary DNA stabilization solution followed by RNase A treatment. Once proteins and other contaminants are removed DNA is precipitated then rehydrated. The high quality extracted DNA is ready for use in a variety of downstream applications.

Specifications (Cat. # GEE150, GEE1.5K, GEE1.5K+)
  • Isolate high molecular weight DNA for archiving or sensitive downstream applications
  • High yield, High quality DNA (A260/A280 = 1.8-2.0)
  • Sample: 0.5-1.5 x 109 bacteria cells
  • Easily scalable to satisfy larger bacteria sample volumes
  • Minimal hands on time
  • Convenient, simple and gentle reagent DNA precipitation method
  • Storage: dry at room temperature (15-25°C) for up to 2 years, Lysozyme is shipped at room temperature and should be stored at -20°C for up to 1 year
  • DNA archiving,
  • PCR,
  • AFLP,
  • Southern Blotting,
  • Real-time PCR
  • Cell Lysis Buffer
  • Gram+ Buffer
  • Protein Removal Buffer
  • DNA Hydration Buffer (10 mM Tris-HCL, 1 mM EDTA, pH8.0)
  • RNase A (10 mg/ml) – not included with GEE1.5K
  • Lysozyme – not included with GEE1.5K
Quality Control

The quality of the Geneaid™ DNA Isolation Kit (Bacteria) is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. Genomic DNA is isolated from 0.5-1.5 x 109 bacteria cells. The isolated DNA (5-15 µg with an A260/A280 ratio of 1.8–2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Reagent Genomic DNA Extraction Kits Specifications
SpecificationsGeneaid™ DNA Isolation Kit BloodGeneaid™ DNA Isolation Kit Cultured CellGeneaid™ DNA Isolation Kit TissueGeneaid™ DNA Isolation Kit BacteriaGeneaid™ DNA Isolation Kit YeastGENEzol™ DNA Reagent Plant
Cat. #GEB100
FormatReagent SystemReagent SystemReagent SystemReagent SystemReagent SystemReagent System
Sample300 µl-10 ml bloodup to 5x107 cultured cellsup to 200 mg tissueup to 1.5x109 bacterial cellsup to 2x108 yeast cellsup to 1 g plant tissue
Elution Volume100 µl-1 ml50-200 µl100-200 µl100 µl50-100 µl50-100 µl
Operation Time<40 minutes<60 minutes<70 minutes<60 minutes<40 minutes<50 minutes
Tablo 1
Product NameRXNSCatalogue Number
Geneaid™ DNA Isolation Kit (Bacteria)150/1,500GEE100/01K/01K+
Geneaid™ DNA Isolation Kit (Blood)100/1,000GEB100/01K/01K+
Geneaid™ DNA Isolation Kit (Tissue)150/1,500GET150/1.5K/1.5K+
Geneaid™ DNA Isolation Kit (Cultured Cell)150/1,500GEC150/1.5K/1.5K+
Geneaid™ DNA Isolation Kit (Yeast)100/300GEY100/300
GENEzol™ DNA Reagent Plant100/200GR100/200