Geneaid™ DNA Isolation Kit (Cultured Cell)
Reagent DNA Extraction/Reagent DNA Extraction
The Geneaid™ DNA Isolation Kit (Cultured Cell) offers a simple and gentle reagent DNA precipitation method for isolating high molecular weight genomic, mitochondrial and viral DNA from cultured cells, suitable for archiving or sensitive downstream applications. This highly versatile solution based system can be scaled proportionately in order to satisfy larger sample volumes providing a convenient sample-storage procedure with minimal hands on time. Initially cells are lysed in the presence of detergents and a proprietary DNA stabilization solution followed by RNase A treatment. Once proteins and other contaminants are removed DNA is precipitated then rehydrated. The high quality extracted DNA is ready for use in a variety of downstream applications.
Specifications (Cat. # GEC150, GEC1.5K, GEC1.5K+)
- Isolate high molecular weight DNA for archiving or sensitive downstream applications
- High yield, High quality DNA (A260/A280 = 1.8-2.0)
- Sample: 0.5-1.5 x 109 cultured cells
- Easily scalable to satisfy larger cultured cell sample volumes
- Minimal hands on time
- Convenient, simple and gentle reagent DNA precipitation method
- Storage: dry at room temperature (15-25ºC) for up to 2 years, RNase A should be stored at -20ºC for extended periods
- DNA archiving,
- PCR,
- AFLP,
- RFLP/PADP,
- Southern Blotting,
- Real-time PCR
Components
- Cell Lysis Buffer
- Protein Removal Buffer
- DNA Hydration Buffer (10 mM Tris-HCL, 1 mM EDTA, pH8.0)
- RNase A (10 mg/ml) – not included with GEC1.5K
Quality Control
The quality of the Geneaid™ DNA Isolation Kit (Cultured Cell) is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. Genomic DNA is isolated from 0.5-1.5 x 109 cultured cells. The isolated DNA (5-15 µg with an A260/A280 ratio of 1.8–2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.
Reagent Genomic DNA Extraction Kits Specifications
| Specifications | Geneaid™ DNA Isolation Kit Blood | Geneaid™ DNA Isolation Kit Cultured Cell | Geneaid™ DNA Isolation Kit Tissue | Geneaid™ DNA Isolation Kit Bacteria | Geneaid™ DNA Isolation Kit Yeast | GENEzol™ DNA Reagent Plant | ||
| Cat. # | GEB100 GEB01K GEB01K+ | GEC150 GEC1.5K GEC1.5K+ | GET150 GET1.5K GET1.5K+ | GEE150 GEE1.5K GEE1.5K+ | GEY100 GEY300 | GR100 GR200 | ||
| RXNS | 100-1,000 | 150-1,500 | 150-1,500 | 150-1,500 | 100-300 | 100-200 | ||
| Format | Reagent System | Reagent System | Reagent System | Reagent System | Reagent System | Reagent System | ||
| Sample | 300 µl-10 ml blood | up to 5x107 cultured cells | up to 200 mg tissue | up to 1.5x109 bacterial cells | up to 2x108 yeast cells | up to 1 g plant tissue | ||
| Elution Volume | 100 µl-1 ml | 50-200 µl | 100-200 µl | 100 µl | 50-100 µl | 50-100 µl | ||
| Operation Time | <40 minutes | <60 minutes | <70 minutes | <60 minutes | <40 minutes | <50 minutes |
| Tablo 1 | ||||
| Product Name | RXNS | Catalogue Number | ||
| Geneaid™ DNA Isolation Kit (Bacteria) | 150/1,500 | GEE100/01K/01K+ | ||
| Geneaid™ DNA Isolation Kit (Blood) | 100/1,000 | GEB100/01K/01K+ | ||
| Geneaid™ DNA Isolation Kit (Tissue) | 150/1,500 | GET150/1.5K/1.5K+ | ||
| Geneaid™ DNA Isolation Kit (Cultured Cell) | 150/1,500 | GEC150/1.5K/1.5K+ | ||
| Geneaid™ DNA Isolation Kit (Yeast) | 100/300 | GEY100/300 | ||
| GENEzol™ DNA Reagent Plant | 100/200 | GR100/200 | ||
