Geneaid™ Maxi Plasmid Kit PLUS (Endotoxin Free)
DNA Extraction/Plasmid DNA Purification
The Geneaid™ Maxi Plasmid Kit PLUS uses pre-packed anion-exchange resin columns to purify plasmid DNA from 200-1100 ml of cultured bacterial cells. This kit includes an increased volume of reagents for processing 2.6-4 g bacteria cell pellets. TrueBlue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. Using an efficient gravity-flow procedure, plasmid DNA is bound to the column and contaminants are efficiently removed. The purified plasmid DNA is eluted then precipitated with isopropanol for desalting. The entire procedure can be completed in 100 minutes without ultracentrifuges, HPLC or other toxic reagents and the purified plasmid DNA is suitable for transfection, sequencing reactions, ligation, PCR, in-vitro transcription, microinjection, restriction enzyme digestion and gene gun.
Specifications (Cat. # PME10+, PME25+)
- Includes an increased volume of reagents for processing 2.6-4 g bacteria cell pellets
- High Yield: 1.0 mg of pure transfection grade plasmid DNA from 300 ml cultured bacterial cells
- Efficient endotoxin removal (<0.1 EU/µg DNA) verified by LAL
- Large Sample Volume: 200-1100 ml of cultured bacterial cells
- Format: plasmid maxiprep anion-exchange resin column
- Elution Volume: 500 µl – 2 ml
- Operation time: within 100 minutes
- Storage: dry at room temperature (15-25ºC)
- Transfection,
- Sequencing,
- Ligation,
- PCR,
- In-vitro Transcription Microinjection,
- Restriction Enzyme Digestion,
- Gene Gun
Components
- PM1, PM2, PM3 Buffer
- TrueBlue Lysis Buffer
- PER Buffer
- PEQ Buffer
- PMC Buffer
- PEL Buffer
- RNase A
- Plasmid Maxi Columns
Quality Control
The quality of the Geneaid™ Maxi Plasmid Kit (Endotoxin Free) is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. Plasmid DNA from a 300 ml overnight E. coli (DH5α) culture, containing plasmid pBluescript (A600 > 2 U/ml) is purified using the Geneaid™ Maxi Plasmid Kit. Following the purification process, a yield of more than 900 μg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid (1 µg) is analyzed by electrophoresis.
Plasmid DNA Midi and Maxi Kits Specifications
| Specifications | Presto™ Midi Plasmid Kit | Presto™ Midi Plasmid Kit Endotoxin Free | Geneaid™ Midi Plasmid Kit | Geneaid™ Midi Plasmid Kit Endotoxin Free | Geneaid™ Maxi Plasmid Kit | Geneaid™ Maxi Plasmid Kit Endotoxin Free | ||
| Cat. # | PIF025 | PIFE25 | PI025 | PIE25 | PM010/25 | PME10/25 | ||
| Format | Anion-Exchange Gravity Flow | Anion-Exchange Gravity Flow | Anion-Exchange Gravity Flow | Anion-Exchange Gravity Flow | Anion-Exchange Gravity Flow | Anion-Exchange Gravity Flow | ||
| Binding Capacity | 350 µg | 350 µg | 350 µg | 350 µg | 1.0 mg | 1.0 mg | ||
| Filter Column | Yes | Yes | N/A | N/A | N/A | N/A | ||
| Endotoxin Level | <0.15 EU/µg | <0.10 EU/µg | <0.15 EU/µg | <0.10 EU/µg | <0.15 EU/µg | <0.10 EU/µg | ||
| Culture Input | 50-150 ml | 50-150 ml | 50-300 ml | 50-300 ml | 200-1600 ml | 200-1600 ml | ||
| Plasmid Size | 1-20 kb | 1-20 kb | 1-20 kb | 1-20 kb | 1-20 kb | 1-20 kb | ||
| Typical Yield | 350 µg | 350 µg | 350 µg | 350 µg | 1.0 mg | 1.0 mg | ||
| Elution Volume | 500 µl-2 ml | 500 µl-2 ml | 500 µl-2 ml | 500 µl-2 ml | 500 µl-2 ml | 500 µl-2 ml | ||
| Operation Time | <80 minutes | <80 minutes | <80 minutes | <80 minutes | <100 minutes | <100 minutes |
| RELATED PRODUCTS | ||
|---|---|---|
| Product Name | RXNS/VOLUME | Catalogue Number |
| Presto™ Endotoxin Free Mini Plasmid Kit | 100 | PEH100 |
| Presto™ Mini Plasmid Kit | 100, 300 | PDH100/300 |
| High-Speed Plasmid Mini Kit | 100, 300 | PD100/300 |
| Large Plasmid DNA Extraction Kit | 100, 300 | PDL100/300 |
| Midiprep Spin Column Plasmid Kit | 25 | PA025 |
| Presto™ Midi Plasmid Kit | 25 | PIF025 |
| Presto™ Midi Plasmid Kit (Endotoxin Free) | 25 | PIFE25 |
| Geneaid™ Midi Plasmid Kit | 25 | PI025 |
| Geneaid™ Midi Plasmid Kit (Endotoxin Free) | 25 | PIE25 |
| Presto™ Plasmid DNA Concentration Kit | 25, 50, 100 | PC0250/500/1000 |
| Geneaid™ Maxi Plasmid Kit | 10, 25 | PM010/25 |
| Geneaid™ Maxi Plasmid Kit (Endotoxin Free) | 10, 25 | PME10/25 |
| Presto™ 96 Well Plasmid Kit | 4 x 96, 10 x 96 | 96PDC04/10 96PDV04/10 |
