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Geneaid™ Midi Plasmid Kit (Endotoxin Free)

DNA Extraction/Plasmid DNA Purification

The Geneaid™ Midi Plasmid Kit Endotoxin Free was designed for plasmid DNA purification and endotoxin removal using pre-packed anion-exchange resin maxiprep columns to purify plasmid DNA from 50-300 ml of cultured bacterial cells. TrueBlue Lysis Buffer (an optional color indicator) is included with the kit in order to prevent common handling errors, ensuring efficient cell lysis and neutralization. This endotoxin free plasmid kit uses a modified alkaline lysis method and RNase treatment to obtain clear cell lysate with minimal genomic DNA/RNA contaminants. Using an efficient gravity-flow procedure, plasmid DNA in the crude lysate is bound to the column and the contaminants are removed with the PMC Buffer. The purified plasmid DNA is eluted by a high salt buffer and then precipitated with Isopropanol for desalting. The entire procedure can be completed in 80 minutes without ultracentrifuges, HPLC or other toxic reagents and the purified plasmid DNA is suitable for Transfection, Sequencing Reactions, Ligation, PCR, and In-vitro Transcription, Microinjection, Restriction Enzyme Digestion and Gene Gun.

Specifications (Cat. # PIE25)

  • High Yield: 350 µg of pure transfection grade plasmid DNA from 100 ml cultured bacterial cells
  • Efficient endotoxin removal (<0.1 EU/µg DNA) verified by LAL
  • Large Sample Volume: 50-300 ml of cultured bacterial cells
  • Format: plasmid maxiprep anion-exchange resin column
  • Elution Volume: 500 µl – 2 ml
  • Operation time: within 80 minutes (only 50 minutes when combined with the Presto™ Plasmid Concentrator)
  • Storage: dry at room temperature (15-25ºC)
  • Transfection,
  • Sequencing,
  • Ligation,
  • PCR,
  • In-vitro Transcription Microinjection,
  • Restriction Enzyme Digestion,
  • Gene Gun


  • PM1, PM2, PM3 Buffer
  • TrueBlue Lysis Buffer
  • PER Buffer
  • PEQ Buffer
  • PMC Buffer
  • PEL Buffer
  • RNase A
  • Plasmid Midi Columns

Quality Control

The quality of the Geneaid™ Midi Plasmid Kit (Endotoxin Free) is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. Plasmid DNA from a 100 ml overnight E. coli (DH5α) culture, containing plasmid pBluescript (A600 > 2 U/ml) is purified using the Geneaid™ Midi Plasmid Kit (Endotoxin Free). Following the purification process, a yield of more than 300 μg is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.

Plasmid DNA Midi and Maxi Kits Specifications

SpecificationsPresto™ Midi Plasmid KitPresto™ Midi Plasmid Kit Endotoxin FreeGeneaid™ Midi Plasmid KitGeneaid™ Midi Plasmid Kit Endotoxin FreeGeneaid™ Maxi Plasmid KitGeneaid™ Maxi Plasmid Kit Endotoxin Free
Cat. #PIF025PIFE25PI025PIE25PM010/25PME10/25
FormatAnion-Exchange Gravity FlowAnion-Exchange Gravity FlowAnion-Exchange Gravity FlowAnion-Exchange Gravity FlowAnion-Exchange Gravity FlowAnion-Exchange Gravity Flow
Binding Capacity350 µg350 µg350 µg350 µg1.0 mg1.0 mg
Filter ColumnYesYesN/AN/AN/AN/A
Endotoxin Level<0.15 EU/µg<0.10 EU/µg<0.15 EU/µg<0.10 EU/µg<0.15 EU/µg<0.10 EU/µg
Culture Input50-150 ml50-150 ml50-300 ml50-300 ml200-1600 ml200-1600 ml
Plasmid Size1-20 kb1-20 kb1-20 kb1-20 kb1-20 kb1-20 kb
Typical Yield350 µg350 µg350 µg350 µg1.0 mg1.0 mg
Elution Volume500 µl-2 ml500 µl-2 ml500 µl-2 ml500 µl-2 ml500 µl-2 ml500 µl-2 ml
Operation Time<80 minutes<80 minutes<80 minutes<80 minutes<100 minutes<100 minutes
Product NameRXNS/VOLUMECatalogue Number
Presto™ Endotoxin Free Mini Plasmid Kit100PEH100
Presto™ Mini Plasmid Kit100, 300PDH100/300
High-Speed Plasmid Mini Kit100, 300PD100/300
Large Plasmid DNA Extraction Kit100, 300PDL100/300
Midiprep Spin Column Plasmid Kit25PA025
Presto™ Midi Plasmid Kit25PIF025
Presto™ Midi Plasmid Kit (Endotoxin Free)25PIFE25
Geneaid™ Midi Plasmid Kit25PI025
Geneaid™ Midi Plasmid Kit (Endotoxin Free)25PIE25
Presto™ Plasmid DNA Concentration Kit25, 50, 100PC0250/500/1000
Geneaid™ Maxi Plasmid Kit10, 25PM010/25
Geneaid™ Maxi Plasmid Kit (Endotoxin Free)10, 25PME10/25
Presto™ 96 Well Plasmid Kit4 x 96, 10 x 9696PDC04/10