Geneius™ Micro gDNA Extraction Kit
DNA Extraction/Genomic DNA Purification
The Geneius™ Micro gDNA Extraction Kit provides an efficient method for purifying DNA (including genomic and mitochondrial DNA) from small volumes of whole blood, dried blood spots (Whatman® FTA® Cards), urine and saliva. This DNA Extraction Kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Carrier RNA is included with this DNA extraction kit to improve the efficiency of DNA binding to the spin column membrane. Contaminants are removed using W1 Buffer and Wash Buffer (containing ethanol). The purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed in as little as 20 minutes without phenol/chloroform extraction or alcohol precipitation, with an average DNA yield of 150-200 ng from 6 mm dried blood spots. Purified DNA, with approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions.
Specifications (Cat. # GMB100, GMB300)
- Purify genomic DNA from small volume blood samples (1 µl), dried blood spots (Whatman® FTA® Cards), urine, saliva
- Time: in as little as 20 minutes
- High Yield: Up to 300 ng of pure genomic DNA per 6 mm diameter punch from Whatman® FTA® Cards (dried blood spots)
- High Purity gDNA: A260/A280 = 1.8-2.0
- DNA Size: 20-30 kb
- Spin Columns: glass fiber membrane optimized for genomic DNA extraction
- Elution volume: 30-100 µl
- Storage: dry at room temperature (15-25°C)
- PCR,
- Restriction Endonuclease Digestions,
- Array,
- Southern Blots and various other enzymatic reactions
Components
- S1 Buffer
- S2 Buffer
- Carrier RNA
- W1 Buffer
- Wash Buffer
- Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
- Proteinase K
- GD Columns
- 2 ml Collection Tubes
Quality Control
The quality of the Geneius™ Micro gDNA Extraction Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. The purified genomic DNA (150-200 ng with an A260/A280 ratio of 1.8-2.0) from 10 µl blood samples is quantified with a spectrophotometer and analyzed by electrophoresis.
Genomic DNA Extraction Kits Specifications
| Specifications | gSYNC™ DNA Extraction Kit | Genomic DNA Mini Kit Blood Cultured Cell | Genomic DNA Mini Kit Tissue | Genomic DNA Mini/Maxi Kit Plant | Presto™ Mini gDNA Bacteria Kit | Presto™ Mini gDNA Yeast Kit | Geneius™ Micro gDNA Extraction Kit | Presto™ Buccal Swab gDNA Extraction Kit | ||
| Cat. # | GS100 GS300 | GB100 GB300 | GT050 GT100 GT300 | GP100 GPM010 GPM025 | GBB100/101 GBB300/301 | GBYB100 GBYB300 | GMB100 GMB300 | GSK100 GSK300 | ||
| Format | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Reagent System | Spin Column | Spin Column | ||
| Binding Capacity | 30 µg | 30 µg | 30 µg | 50 µg (Mini) 500 µg (Maxi) | 50 µg | N/A | 50 µg | 50 µg | ||
| Sample | 200 µl blood | 300 µl blood | 30 mg animal tissue | Mini: 100 mg plant tissue | 1x109 bacterial cells | 2x108 yeast cells | 1-100 µl blood | buccal swabs | ||
| 25 mg animal tissue | 200 µl buffy coat | Maxi: 1 g plant tissue | 200 µl blood | FTA® Cards | ||||||
| 1x107 cultured cells | 1x107 cultured cells | 1 ml urine, plasma | 1 ml urine | |||||||
| hair | 2x108 yeast/fungus | 1-100 µl saliva | ||||||||
| Micropestle | N/A | N/A | Yes | N/A | N/A | N/A | N/A | N/A | ||
| Filter Column | N/A | N/A | N/A | Yes | N/A | N/A | N/A | Yes | ||
| Enzyme | Proteinase K | N/A | Proteinase K | N/A | Lysozyme Proteinase K | Lyticase or Zymolase | Proteinase K | Proteinase K | ||
| Typical Yield | 5 µg from 200 µl blood | 4-10 µg from 300 µl blood | 10-40 µg from 20 mg tissue | 3-5 µg from 100 mg Arabidopsis leaf (Mini) | 37 µg from 1x109 E. coli | 10-15 µg from 2x108 S. cerevisiae cells | 300 ng from 6 mm blood spots | 2 µg per swab | ||
| 10-40 µg from 20 mg tissue | 80 µg from 500 mg Lemon leaf (Maxi) | 10-15 µg from 1x109 Bacillus subtilis | ||||||||
| Elution Volume | 30-100 µl | 30-200 µl | 30-200 µl | 30-200 µl (Mini) 1 ml (Maxi) | 30-200 µl | 50-100 µl | 30-100 µl | 50-100 µl | ||
| Operation Time | <20 minutes | <25 minutes | 30 minutes | <30 minutes | <30 minutes | <40 minutes | <20 minutes | <20 minutes |
| Product Name | RXNS | Catalogue Number | ||
| gSYNC™ DNA Extraction Kit (Tissue/Blood) | 100, 300 | GS100/300 | ||
| Genomic DNA Mini Kit (Blood/Cultured Cell) | 100, 300 | GB100/300 | ||
| Genomic DNA Maxi Kit (Plant) | 10, 25 | GPM010/25 | ||
| Genomic DNA Maxi Kit (Blood/Cultured Cell) | 10, 25 | GDM10/25 | ||
| Genomic DNA Mini Kit (Tissue) | 50, 100, 300 | GT050/100/300 | ||
| Genomic DNA Mini Kit (Plant) | 100 | GP100 | ||
| Geneius™ Micro gDNA Extraction Kit | 100 | GMB100/300 | ||
| Presto™ Buccal Swab gDNA Extraction Kit | 100 | GSK100/300 | ||
| Presto™ Mini gDNA Yeast Kit | 100, 300 | GBYB100/300 | ||
| Presto™ Mini gDNA Bacteria Kit | 100, 300 | GBB100/101/300/301 | ||
| Presto™ 96 Well Blood Genomic DNA Extraction Kit | 4 x 96, 10 x 96 | 96GBP04/10 | ||
| Presto™ 96 Well Plant Genomic DNA Extraction Kit | 4 x 96, 10 x 96 | 96GPP04/10 | ||
| Presto™ 96 Well gDNA Bacteria Kit | 4 x 96, 10 x 96 | 96GBB04/10 |
