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GENEzol™ TriRNA Bacteria Kit without DNase

RNA Extraction/Tri RNA

The GENEzol™ TriRNA Bacteria Kit is a phenol and guanidine isothiocyanate plus spin column system for convenient purification of high-quality total RNA from bacteria samples. Bacterial cell walls are initially lysed using Lysozyme. The sample is then homogenized in GENEzol™ Reagent without chloroform phase separation or isopropanol RNA precipitation. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of sensitive downstream applications.

Advantages (Cat. # GZB050, GZB100, GZB200)
  • Purify total RNA within 20 minutes without chloroform phase separation or isopropanol RNA precipitation
  • Sample Volume: bacteria cells up to 1 x 109
  • Format: phenol, chlorform, guanidine isothiocyanate plus spin column system
  • High quality RNA: A260/A280 >1.8, A260/A230 >1.8
  • Storage: GENEzol™ Reagent should be stored at 2ºC to 25ºC, Lysozyme can be shipped at room temperature but should be stored at -20ºC for extended periods
  • cDNA Library Construction,
  • Cloning,
  • RT-PCR (Endpoint),
  • Real-Time PCR,
  • Nuclease Protection Assays,
  • Northern Blotting
Components
  • GENEzol™ Reagent
  • Pre-Wash Buffer
  • Lysozyme
  • Bacteria Lysis Buffer
  • Wash Buffer
  • RNase-free Water
  • RB Columns
  • 2 ml Collection Tubes
Quality Control

The GENEzol™ TriRNA Bacteria Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. 5 µl from a 50 µl eluate of purified RNA is analyzed by electrophoresis on a 1% agarose gel.

Product NameRXNSCatalogue Number
GENEzol™ Reagent50/100/200GZR050/100/200
GENEzol™ TriRNA Pure Kit50/100/200GZX050/100/200, GZXD050/100/200
GENEzol™ TriRNA Bacteria Kit50/100GZB050/100
TriRNA Pure Kit50/100/200TRP050/100/200, TRPD050/100/200
RNA Cleanup Kit50/100PR050/100
GENEzol™ 96 Well TriRNA Pure Kit4 x 96/10 x 9696GZX04/10