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Genomic DNA Midi Kit (Blood)

DNA Extraction/Genomic DNA Purification

The Genomic DNA Midi Kit Blood is optimized for DNA purification (including genomic, mitochondrial and viral DNA) from up to 3 ml of fresh blood using an efficient mini spin column and column extension tube system. This DNA Extraction Kit uses Protease to reduce hemoglobin contamination while chaotropic salt and detergents are used to lyse cells and degrade protein, allowing the DNA to bind to the glass fiber matrix of the genomic DNA spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. The entire procedure can be completed 30 minutes without phenol/chloroform extraction or alcohol precipitation. Purified DNA, with approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions.

Specifications (Cat. # GDI100)
  • Binding Capacity: up to 50 μg
  • Large Sample Size: up to 3 ml of blood compared to 200 µl using the mini kit
  • Genomic glass fiber mini spin column and Column Extension Tubes for increased sample volume and yield
  • Elution Volume: 30-100 µl
  • Storage: dry at room temperature (15-25°C)
  • Operation time: within 30 minutes
  • PCR,
  • RFLP,
  • Southern Blotting,
  • Real-time PCR
  • GSB Buffer
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • Protease
  • GS Columns and Column Extension Tubes
  • 2 ml Collection Tubes
Quality Control

The quality of the Genomic DNA Midi Kit (Blood) is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. Genomic DNA from 3 ml of whole human blood is extracted using the Genomic DNA Midi Kit (Blood). The purified DNA is quantified with a spectrophotometer and analyzed by electrophoresis.

Product NameRXNSCatalogue Number
gSYNC™ DNA Extraction Kit (Tissue/Blood)100, 300GS100/300
Genomic DNA Mini Kit (Blood/Cultured Cell)100, 300GB100/300
Genomic DNA Maxi Kit (Plant)10, 25GPM010/25
Genomic DNA Maxi Kit (Blood/Cultured Cell)10, 25GDM10/25
Genomic DNA Mini Kit (Tissue)50, 100, 300GT050/100/300
Genomic DNA Mini Kit (Plant)100GP100
Geneius™ Micro gDNA Extraction Kit100GMB100/300
Presto™ Buccal Swab gDNA Extraction Kit100GSK100/300
Presto™ Mini gDNA Yeast Kit100, 300GBYB100/300
Presto™ Mini gDNA Bacteria Kit100, 300GBB100/101/300/301
Presto™ 96 Well Blood Genomic DNA Extraction Kit4 x 96, 10 x 9696GBP04/10
Presto™ 96 Well Plant Genomic DNA Extraction Kit4 x 96, 10 x 9696GPP04/10
Presto™ 96 Well gDNA Bacteria Kit4 x 96, 10 x 9696GBB04/10