Genomic DNA Mini Kit (Blood / Cultured Cell)
DNA Extraction/Genomic DNA Purification
The Genomic DNA Mini Kit (Blood/Cultured Cell) is a genomic DNA extraction kit optimized for genomic, mitochondrial and virus DNA purification from whole blood, buffy coat and cultured cell samples. This Genomic DNA Extraction Kit uses RBC Lysis Buffer and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the genomic DNA spin column. Contaminants are removed using a Wash Buffer (containing ethanol) and the purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. Phenol/chloroform extraction or alcohol precipitation is not required and the purified genomic DNA (approximately 20-30 kb) is suitable for use in PCR or various other enzymatic reactions. For RNA purification from blood and cultured cell samples, the Total RNA Mini Kit (Blood/CC) is also available.
Specifications (Cat. # GB100, GB300)
- Purify genomic DNA within 25 minutes!
- High Yield, High Quality Genomic DNA: 4-10 μg of pure genomic DNA (A260/A280 = 1.8-2.0) from 300 µl of whole blood
- Variety of Samples: up to 300 µl of whole blood, up to 200 µl of buffy coat, up to 1 x 107 cultured cells, up to 2 x 108 yeast and other fungus species
- Spin Columns: glass fiber membrane optimized for genomic DNA extraction
- Elution Volume: 30-100 µl
- Storage: dry at room temperature (15-25°C)
- PCR,
- AFLP/PADP,
- RFLP,
- Southern Blotting,
- Real-time PCR
Components
- RBC Lysis Buffer
- GT Buffer
- GB Buffer
- W1 Buffer
- Wash Buffer
- Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
- GD Columns
- 2 ml Collection Tubes
Quality Control
The quality of the Genomic DNA Mini Kit (Blood/Cultured Cell) is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. Genomic DNA from 300 μl of whole human blood is extracted using the Genomic DNA Mini Kit (Blood/Cultured Cell). The purified DNA (4-10 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.
Genomic DNA Extraction Kits Specifications
| Specifications | gSYNC™ DNA Extraction Kit | Genomic DNA Mini Kit Blood Cultured Cell | Genomic DNA Mini Kit Tissue | Genomic DNA Mini/Maxi Kit Plant | Presto™ Mini gDNA Bacteria Kit | Presto™ Mini gDNA Yeast Kit | Geneius™ Micro gDNA Extraction Kit | Presto™ Buccal Swab gDNA Extraction Kit | ||
| Cat. # | GS100 GS300 | GB100 GB300 | GT050 GT100 GT300 | GP100 GPM010 GPM025 | GBB100/101 GBB300/301 | GBYB100 GBYB300 | GMB100 GMB300 | GSK100 GSK300 | ||
| Format | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Reagent System | Spin Column | Spin Column | ||
| Binding Capacity | 30 µg | 30 µg | 30 µg | 50 µg (Mini) 500 µg (Maxi) | 50 µg | N/A | 50 µg | 50 µg | ||
| Sample | 200 µl blood | 300 µl blood | 30 mg animal tissue | Mini: 100 mg plant tissue | 1x109 bacterial cells | 2x108 yeast cells | 1-100 µl blood | buccal swabs | ||
| 25 mg animal tissue | 200 µl buffy coat | Maxi: 1 g plant tissue | 200 µl blood | FTA® Cards | ||||||
| 1x107 cultured cells | 1x107 cultured cells | 1 ml urine, plasma | 1 ml urine | |||||||
| hair | 2x108 yeast/fungus | 1-100 µl saliva | ||||||||
| Micropestle | N/A | N/A | Yes | N/A | N/A | N/A | N/A | N/A | ||
| Filter Column | N/A | N/A | N/A | Yes | N/A | N/A | N/A | Yes | ||
| Enzyme | Proteinase K | N/A | Proteinase K | N/A | Lysozyme Proteinase K | Lyticase or Zymolase | Proteinase K | Proteinase K | ||
| Typical Yield | 5 µg from 200 µl blood | 4-10 µg from 300 µl blood | 10-40 µg from 20 mg tissue | 3-5 µg from 100 mg Arabidopsis leaf (Mini) | 37 µg from 1x109 E. coli | 10-15 µg from 2x108 S. cerevisiae cells | 300 ng from 6 mm blood spots | 2 µg per swab | ||
| 10-40 µg from 20 mg tissue | 80 µg from 500 mg Lemon leaf (Maxi) | 10-15 µg from 1x109 Bacillus subtilis | ||||||||
| Elution Volume | 30-100 µl | 30-200 µl | 30-200 µl | 30-200 µl (Mini) 1 ml (Maxi) | 30-200 µl | 50-100 µl | 30-100 µl | 50-100 µl | ||
| Operation Time | <20 minutes | <25 minutes | 30 minutes | <30 minutes | <30 minutes | <40 minutes | <20 minutes | <20 minutes |
| Product Name | RXNS | Catalogue Number | ||
| gSYNC™ DNA Extraction Kit (Tissue/Blood) | 100, 300 | GS100/300 | ||
| Genomic DNA Mini Kit (Blood/Cultured Cell) | 100, 300 | GB100/300 | ||
| Genomic DNA Maxi Kit (Plant) | 10, 25 | GPM010/25 | ||
| Genomic DNA Maxi Kit (Blood/Cultured Cell) | 10, 25 | GDM10/25 | ||
| Genomic DNA Mini Kit (Tissue) | 50, 100, 300 | GT050/100/300 | ||
| Genomic DNA Mini Kit (Plant) | 100 | GP100 | ||
| Geneius™ Micro gDNA Extraction Kit | 100 | GMB100/300 | ||
| Presto™ Buccal Swab gDNA Extraction Kit | 100 | GSK100/300 | ||
| Presto™ Mini gDNA Yeast Kit | 100, 300 | GBYB100/300 | ||
| Presto™ Mini gDNA Bacteria Kit | 100, 300 | GBB100/101/300/301 | ||
| Presto™ 96 Well Blood Genomic DNA Extraction Kit | 4 x 96, 10 x 96 | 96GBP04/10 | ||
| Presto™ 96 Well Plant Genomic DNA Extraction Kit | 4 x 96, 10 x 96 | 96GPP04/10 | ||
| Presto™ 96 Well gDNA Bacteria Kit | 4 x 96, 10 x 96 | 96GBB04/10 |
