miRNA Isolation Kit
RNA Extraction/Total RNA
The miRNA Isolation Kit provides a quick and easy spin column system for purifying and enriching micro RNAs (miRNAs) and other small cellular RNAs from a wide variety of tissue and cells. Since miRNAs are vital for regulating gene expression, this kit is optimized for isolation of small RNA molecules while removing larger RNAs and minimizing genomic DNA contamination for improved sensitive downstream applications.
Specifications (Cat. # RMI050)
- Purify miRNA within 30 minutes!
- High yield of miRNA and other small RNA
- Efficient removal of larger RNA for sensitive downstream applications
- Sample: 100 mg of tissue, up to 1 x 106 cultured cells
- Spin Columns: glass fiber membrane optimized for miRNA extraction
- Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
- Micropestle: certified RNase and DNase-free
- Storage: dry at room temperature (15-25ºC) for up to 9 months
- miRNA analysis,
- siRNA analysis,
- shRNA analysis,
- snRNA analysis,
- qPCR,
- microarray analysis
Components
- Lysis Buffer
- Mi Buffer
- Wash Buffer
- Release Buffer
- RNA Columns
- 2 ml Collection Tubes
- Micropestles
Quality Control
The quality of the miRNA Isolation Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. 1/5 volume aliquots are analyzed on a polyacrylamide gel. The majority of RNA visible on the gel are <100 nt in size, with the major bands corresponding to tRNAs. The 5S and 5.8S rRNA species may also be visible. These tRNA and small rRNA bands should be clear and distinct. miRNA (21-22 nt) are typically not visible on the gel image.
Total RNA Extraction Kits Specifications
| Specifications | Total RNA Mini Kit Blood Cultured Cell | Total RNA Mini Kit Tissue | Total RNA Mini Kit Plant | Presto™ Mini RNA Bacteria Kit | Presto™ Mini RNA Yeast Kit | miRNA Isolation Kit | GENEzol™ TriRNA Pure Kit | ||
| Cat. # | RB050 RB100 RB300 | RT050 RT100 RT300 | RP050 RP100 RP300 | RBB050 RBB100 RBB300 | RBY050 RBY100 RBY300 | RMI050 | GZX050 GZX100 GZX200 | ||
| Format | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Tri Reagent Spin Column | ||
| Binding Capacity | 50 µg | 50 µg | 50 µg | 50 µg | 50 µg | 50 µg | 50 µg | ||
| Sample | blood, cells | animal tissue | plant tissue | bacterial cells | yeast cells | animal tissue, cells | blood,cells, tissue, body fluids, bacteria | ||
| Micropestle | N/A | Yes | N/A | N/A | N/A | Yes | N/A | ||
| Filter Column | N/A | Yes | Yes | N/A | N/A | N/A | N/A | ||
| Enzyme | N/A | N/A | N/A | Lysozyme | N/A | N/A | N/A | ||
| Typical Yield | 2-3 µg from 300 µl blood, 25-30 µg from 106 cells | 5-30 µg | 5-30 µg | 40-50 µg from 1x109 bacterial cells | 15-20 µg from 5x107 yeast cells | 5-30 µg | 5-30 µg | ||
| Elution Volume | 50 µl | 50 µl | 50 µl | 50 µl | 50 µl | 50 µl | 25-50 µl | ||
| Time | <20 min. | <15 min. | <20 min. | <20 min. | <20 min. | <30 min. | <15 min. |
| Product Name | RXNS | Catalogue Number | ||
| Total RNA Mini Kit (Blood/Cultured Cell) | 50/100/300 | RB050/100/300, RBD050/100/300 | ||
| Total RNA Mini Kit (Tissue) | 50/100/300 | RT050/100/300, RTD050/100/300 | ||
| Total RNA Mini Kit (Plant) | 50/100/300 | RP050/100/300, RPD050/100/300 | ||
| Presto™ Mini RNA Bacteria Kit | 50/100/300 | RBB050/100/300, RBBD050/100/300 | ||
| Presto™ Mini RNA Yeast Kit | 50/100/300 | RBY050/100/300, RBYD050/100/300 | ||
| miRNA Isolation Kit | 50 | RMI050 | ||
| GENEzol™ TriRNA Pure Kit | 50/100/200 | GZX050/100/200, GZXD050/100/200 | ||
| GENEzol™ 96 Well TriRNA Pure Kit | 4 x 96/10 x 96 | 96GZX04/10 |
