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miRNA Isolation Kit

RNA Extraction/Total RNA

The miRNA Isolation Kit provides a quick and easy spin column system for purifying and enriching micro RNAs (miRNAs) and other small cellular RNAs from a wide variety of tissue and cells. Since miRNAs are vital for regulating gene expression, this kit is optimized for isolation of small RNA molecules while removing larger RNAs and minimizing genomic DNA contamination for improved sensitive downstream applications.

Specifications (Cat. # RMI050)
  • Purify miRNA within 30 minutes!
  • High yield of miRNA and other small RNA
  • Efficient removal of larger RNA for sensitive downstream applications
  • Sample: 100 mg of tissue, up to 1 x 106 cultured cells
  • Spin Columns: glass fiber membrane optimized for miRNA extraction
  • Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
  • Micropestle: certified RNase and DNase-free
  • Storage: dry at room temperature (15-25ºC) for up to 9 months
  • miRNA analysis,
  • siRNA analysis,
  • shRNA analysis,
  • snRNA analysis,
  • qPCR,
  • microarray analysis
  • Lysis Buffer
  • Mi Buffer
  • Wash Buffer
  • Release Buffer
  • RNA Columns
  • 2 ml Collection Tubes
  • Micropestles
Quality Control

The quality of the miRNA Isolation Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. 1/5 volume aliquots are analyzed on a polyacrylamide gel. The majority of RNA visible on the gel are <100 nt in size, with the major bands corresponding to tRNAs. The 5S and 5.8S rRNA species may also be visible. These tRNA and small rRNA bands should be clear and distinct. miRNA (21-22 nt) are typically not visible on the gel image.

Total RNA Extraction Kits Specifications
SpecificationsTotal RNA Mini Kit Blood Cultured CellTotal RNA Mini Kit TissueTotal RNA Mini Kit PlantPresto™ Mini RNA Bacteria KitPresto™ Mini RNA Yeast KitmiRNA Isolation KitGENEzol™ TriRNA Pure Kit
Cat. #RB050
FormatSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnTri Reagent Spin Column
Binding Capacity50 µg50 µg50 µg50 µg50 µg50 µg50 µg
Sampleblood, cellsanimal tissueplant tissuebacterial cellsyeast cellsanimal tissue, cellsblood,cells,
tissue, body fluids, bacteria
Filter ColumnN/AYesYesN/AN/AN/AN/A
Typical Yield2-3 µg from 300 µl blood, 25-30 µg from 106 cells5-30 µg5-30 µg40-50 µg from 1x109 bacterial cells15-20 µg from 5x107 yeast cells5-30 µg5-30 µg
Elution Volume50 µl50 µl50 µl50 µl50 µl50 µl25-50 µl
Time<20 min.<15 min.<20 min.<20 min.<20 min.<30 min.<15 min.
Product NameRXNSCatalogue Number
Total RNA Mini Kit (Blood/Cultured Cell)50/100/300RB050/100/300, RBD050/100/300
Total RNA Mini Kit (Tissue)50/100/300RT050/100/300, RTD050/100/300
Total RNA Mini Kit (Plant)50/100/300RP050/100/300, RPD050/100/300
Presto™ Mini RNA Bacteria Kit50/100/300RBB050/100/300, RBBD050/100/300
Presto™ Mini RNA Yeast Kit50/100/300RBY050/100/300, RBYD050/100/300
miRNA Isolation Kit50RMI050
GENEzol™ TriRNA Pure Kit50/100/200GZX050/100/200, GZXD050/100/200
GENEzol™ 96 Well TriRNA Pure Kit4 x 96/10 x 9696GZX04/10