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Presto™ 96 Well PCR Cleanup Kit

DNA Extraction/PCR Cleanup

Presto™ 96 Well PCR Cleanup Kits were designed for DNA fragments purification from PCR or other enzymatic reactions using an efficient 96 well binding plate system. This PCR Purification Kit uses chaotropic salt to denature enzymes, allowing DNA fragments to be bound by the glass fiber matrix in each well of the 96 well binding plate. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation and the purified DNA is ready for use in subsequent reactions. The Presto™ 96 Well PCR Cleanup Kit is a cost effective, high performance alternative for efficient DNA fragments PCR purification.

Specifications (Cat. # 96DFH04, 96DFH10)
  • Purify up to 300 mg of agarose gel slices per well in 45 minutes!
  • Purify up to 100 µl of PCR products per well in 20 minutes!
  • High Recovery: up to 95%
  • Format: Presto™ PCR Cleanup 96 Well Binding Plates
  • Broad Fragment Size Range: 70 bp–20 kb
  • Centrifuge or vacuum manifold protocol
  • Elution Volume: 60 µl from 80 µl elution buffer volume, 40 µl from 60 µl elution buffer volume
  • Storage: dry at room temperature (15-25ºC)
  • PCR,
  • Fluorescent or Radioactive Sequencing,
  • Restriction Enzyme Digestion,
  • DNA Labeling,
  • Ligation
  • Binding Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • Presto™ PCR Cleanup 96 Well Binding Plates
  • 0.35 ml Collection Plates
  • Adhesive Film
Quality Control

The quality of the Presto™ 96 Well PCR Cleanup Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. DNA fragments of various sizes are purified from PCR products and analyzed by electrophoresis.

Presto™ 96 Well PCR Cleanup Kit Interactive Protocol

Gel Extraction & PCR Cleanup Kits Specifications
SpecificationsGenepHlow™ Gel Extraction KitGenepHlow™ PCR Cleanup KitGenepHlow™ Gel/PCR KitGenepHlow™ DNA Cleanup Maxi KitPresto™ 96 Well PCR Cleanup KitSmall DNA Fragments Extraction KitPresto™ Max Gel/PCR Kit
Cat. #DFG100
FormatSpin ColumnSpin ColumnSpin ColumnMaxi Spin ColumnSpin ColumnSpin ColumnSilica Matrix
Binding Capacity10 µg10 µg10 µg200 µg10 µg10 µg5 µg
SampleAgarose Gel SlicePCR ProductAgarose Gel Slice/PCR ProductAgarose Gel Slice/Restriction Enzyme Digest ProductPCR ProductAgarose Gel Slice/PCR ProductAgarose Gel Slice/PCR Product
Fragment Size70 bp-20 kb70 bp-20 kb70 bp-20 kb100 bp-10 kb70 bp-20 kb40 bp-200 bp100 bp-50 kb
pH IndicatorYesYesYesYesN/AN/AYes
RecoveryUp to 90%Up to 95%Up to 95%Up to 80%Up to 95%Up to 95%Up to 95% (<10 kb)
Up to 80% (10-50 kb)
Elution Volume20-50 µl20-50 µl20-50 µl500-1000 µl60-80 µl20-50 µl10-20 µl
Operation Time<20 minutes<10 minutes<20 minutes<25 minutes<20 minutes<20 minutes<20 minutes
Product NameRXNSCatalogue Number
GenepHlow™ Gel Extraction Kit100/300DFG100/300
GenepHlow™ PCR Cleanup Kit100/300DFC100/300
GenepHlow™ Gel/PCR Kit100/300DFH100/300
GenepHlow™ DNA Cleanup Maxi Kit10/25DFM010/25
Presto™ 96 Well PCR Cleanup Kit4 x 96/10 x 9696DFH04/10
Small DNA Fragments Extraction Kit100/300DF101/301
Presto™ Max Gel/PCR Kit100/300DFL100/300
Gel/PCR DNA Fragments Extraction Kit100/300DF100/300
Presto™ 96 Well Gel Extraction Kit4 x 96/10 x 9696DFG04/10