Presto™ 96 Well Plasmid Kit
DNA Extraction/Plasmid DNA Purification
The Presto™ 96 Well Plasmid Kit was designed for high-throughput purification of plasmid DNA from 1-5 ml of cultured bacterial cells per well using an efficient 96 well filter plate and 96 well binding plate system. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 16-20 μg for high-copy number plasmid or 3-10 μg for low-copy number plasmid from 4 ml of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 40 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.
Specifications (Cat. # 96PDV04, 96PDV10)
- Purify 96 wells of pure plasmid DNA in 40 minutes!
- Sample Volume: 1-2 ml of cultured bacterial cells (OD600=2-3) per well with using Filter Plate/ 1-5 ml of cultured bacterial cells (OD600=2-3) per well without using Filter Plate
- Yield: 4-6 μg/ml of cultured bacterial cells (high-copy plasmid)
- Binding Capacity: 35 μg per well
- Plasmid Size Range: 1-20 kb
- 96 Well plates for filtration and DNA binding
- Elution Volume: 60 μl from 100 μl elution buffer volume
- Centrifuge or vacuum manifold protocol
- Storage: dry at room temperature (15-25ºC)
- Fluorescent and Radioactive Sequencing,
- Restriction Enzyme Digestion,
- Library Screening,
- Ligation,
- Transformation,
- PCR
Components
- P1, P2, P3 Buffers
- Wash Buffer
- Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
- RNase A
- Presto™ Plasmid 96 Well Filter Plates
- Presto™ Plasmid 96 Well Binding Plates
- 0.35 ml Collection Plates
- Adhesive Film
Quality Control
The quality of the Presto™ 96 Well Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 ml overnight E. coli (DH5α) culture containing plasmid pBluescript (OD600=2-3). Following the purification process, a yield of more than 16 μg per well is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.
Presto™ 96 Well Plasmid Kit Interactive Protocol
Plasmid DNA Kit Specifications
| Specifications | Presto™ Midi Plasmid Kit | Presto™ Midi Plasmid Kit Endotoxin Free | Geneaid™ Midi Plasmid Kit | Geneaid™ Midi Plasmid Kit Endotoxin Free | Geneaid™ Maxi Plasmid Kit | Geneaid™ Maxi Plasmid Kit Endotoxin Free | ||
| Cat. # | PIF025 | PIFE25 | PI025 | PIE25 | PM010/25 | PME10/25 | ||
| Format | Anion-Exchange Gravity Flow | Anion-Exchange Gravity Flow | Anion-Exchange Gravity Flow | Anion-Exchange Gravity Flow | Anion-Exchange Gravity Flow | Anion-Exchange Gravity Flow | ||
| Binding Capacity | 350 µg | 350 µg | 350 µg | 350 µg | 1.0 mg | 1.0 mg | ||
| Filter Column | Yes | Yes | N/A | N/A | N/A | N/A | ||
| Endotoxin Level | <0.15 EU/µg | <0.10 EU/µg | <0.15 EU/µg | <0.10 EU/µg | <0.15 EU/µg | <0.10 EU/µg | ||
| Culture Input | 50-150 ml | 50-150 ml | 50-300 ml | 50-300 ml | 200-1600 ml | 200-1600 ml | ||
| Plasmid Size | 1-20 kb | 1-20 kb | 1-20 kb | 1-20 kb | 1-20 kb | 1-20 kb | ||
| Typical Yield | 350 µg | 350 µg | 350 µg | 350 µg | 1.0 mg | 1.0 mg | ||
| Elution Volume | 500 µl-2 ml | 500 µl-2 ml | 500 µl-2 ml | 500 µl-2 ml | 500 µl-2 ml | 500 µl-2 ml | ||
| Operation Time | <80 minutes | <80 minutes | <80 minutes | <80 minutes | <100 minutes | <100 minutes |
| RELATED PRODUCTS | ||
|---|---|---|
| Product Name | RXNS/VOLUME | Catalogue Number |
| Presto™ Endotoxin Free Mini Plasmid Kit | 100 | PEH100 |
| Presto™ Mini Plasmid Kit | 100, 300 | PDH100/300 |
| High-Speed Plasmid Mini Kit | 100, 300 | PD100/300 |
| Large Plasmid DNA Extraction Kit | 100, 300 | PDL100/300 |
| Midiprep Spin Column Plasmid Kit | 25 | PA025 |
| Presto™ Midi Plasmid Kit | 25 | PIF025 |
| Presto™ Midi Plasmid Kit (Endotoxin Free) | 25 | PIFE25 |
| Geneaid™ Midi Plasmid Kit | 25 | PI025 |
| Geneaid™ Midi Plasmid Kit (Endotoxin Free) | 25 | PIE25 |
| Presto™ Plasmid DNA Concentration Kit | 25, 50, 100 | PC0250/500/1000 |
| Geneaid™ Maxi Plasmid Kit | 10, 25 | PM010/25 |
| Geneaid™ Maxi Plasmid Kit (Endotoxin Free) | 10, 25 | PME10/25 |
| Presto™ 96 Well Plasmid Kit | 4 x 96, 10 x 96 | 96PDC04/10 96PDV04/10 |
