Presto™ 96 Well Plasmid Kit without Filter Plate

DNA Extraction/Plasmid DNA Purification

The Presto™ 96 Well Plasmid Kit was designed for high-throughput purification of plasmid DNA from 1-5 ml of cultured bacterial cells per well using an efficient 96 well binding plate system. A modified alkaline lysis method and RNase treatment are used to obtain clear cell lysate with minimal genomic DNA and RNA contaminants. Typical yields are 16-20 μg for high-copy number plasmid or 3-10 μg for low-copy number plasmid from 4 ml of cultured bacterial cells. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 60 minutes. The purified plasmid DNA is ready for use in restriction enzyme digestion, ligation, PCR, and sequencing reactions.

Specifications (Cat. # 96PDC04, 96PDC10)

Purify 96 wells of pure plasmid DNA in 60 minutes!
Sample Volume: 1-5 ml of cultured bacterial cells per well
Yield: 16-20 μg per well (high-copy plasmid), 3-10 μg per well (low-copy plasmid)
Binding Capacity: 35 μg per well
Plasmid Size Range: 1-20 kb
96 Well plates for plasmid DNA binding
Elution Volume: 60 μl from 100 μl elution buffer volume
Storage: dry at room temperature (15-25ºC)

Fluorescent and Radioactive Sequencing,
Restriction Enzyme Digestion,
Library Screening,
Ligation,
Transformation,
PCR

Components

P1, P2, P3 Buffers
Wash Buffer
Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
RNase A
Presto™ Plasmid 96 Well Binding Plates
96 Deep Well Plates
0.35 ml Collection Plates
Adhesive Film

Quality Control

The quality of the Presto™ 96 Well Plasmid Kit is tested on a lot-to-lot basis by isolating plasmid DNA from a 4 ml overnight E. coli (DH5α) culture containing plasmid pBluescript (OD₆₀₀=2-3). Following the purification process, a yield of more than 16 μg per well is obtained and the A260/A280 ratio is between 1.8-2.0. The purified plasmid DNA (1 µg) is used in EcoRI digestion, and analyzed by electrophoresis.

Presto™ 96 Well Plasmid Kit Interactive Protocol

Plasmid DNA Kit Specifications

Specifications	Presto™ Midi Plasmid Kit	Presto™ Midi Plasmid Kit Endotoxin Free	Geneaid™ Midi Plasmid Kit	Geneaid™ Midi Plasmid Kit Endotoxin Free	Geneaid™ Maxi Plasmid Kit	Geneaid™ Maxi Plasmid Kit Endotoxin Free
Cat. #	PIF025	PIFE25	PI025	PIE25	PM010/25	PME10/25
Format	Anion-Exchange Gravity Flow	Anion-Exchange Gravity Flow	Anion-Exchange Gravity Flow	Anion-Exchange Gravity Flow	Anion-Exchange Gravity Flow	Anion-Exchange Gravity Flow
Binding Capacity	350 µg	350 µg	350 µg	350 µg	1.0 mg	1.0 mg
Filter Column	Yes	Yes	N/A	N/A	N/A	N/A
Endotoxin Level	<0.15 EU/µg	<0.10 EU/µg	<0.15 EU/µg	<0.10 EU/µg	<0.15 EU/µg	<0.10 EU/µg
Culture Input	50-150 ml	50-150 ml	50-300 ml	50-300 ml	200-1600 ml	200-1600 ml
Plasmid Size	1-20 kb	1-20 kb	1-20 kb	1-20 kb	1-20 kb	1-20 kb
Typical Yield	350 µg	350 µg	350 µg	350 µg	1.0 mg	1.0 mg
Elution Volume	500 µl-2 ml	500 µl-2 ml	500 µl-2 ml	500 µl-2 ml	500 µl-2 ml	500 µl-2 ml
Operation Time	<80 minutes	<80 minutes	<80 minutes	<80 minutes	<100 minutes	<100 minutes

Presto 96 Well Plasmid Kit

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Product Name	RXNS/VOLUME	Catalogue Number
Presto™ Endotoxin Free Mini Plasmid Kit	100	PEH100
Presto™ Mini Plasmid Kit	100, 300	PDH100/300
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Large Plasmid DNA Extraction Kit	100, 300	PDL100/300
Midiprep Spin Column Plasmid Kit	25	PA025
Presto™ Midi Plasmid Kit	25	PIF025
Presto™ Midi Plasmid Kit (Endotoxin Free)	25	PIFE25
Geneaid™ Midi Plasmid Kit	25	PI025
Geneaid™ Midi Plasmid Kit (Endotoxin Free)	25	PIE25
Presto™ Plasmid DNA Concentration Kit	25, 50, 100	PC0250/500/1000
Geneaid™ Maxi Plasmid Kit	10, 25	PM010/25
Geneaid™ Maxi Plasmid Kit (Endotoxin Free)	10, 25	PME10/25
Presto™ 96 Well Plasmid Kit	4 x 96, 10 x 96	96PDC04/10 96PDV04/10