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Presto™ Buccal Swab gDNA Extraction Kit

DNA Extraction/Genomic DNA Purification

The Presto™ Buccal Swab gDNA Extraction Kit provides an efficient method for purifying DNA (including genomic, mitochondrial and viral DNA) from buccal cells. This DNA Extraction Kit uses Proteinase K and chaotropic salt to lyse cells and degrade protein, allowing DNA to bind to the glass fiber matrix of the spin column. Carrier RNA is included with the kit to improve the efficiency of DNA binding to the spin column membrane. Contaminants are removed using W1 Buffer and Wash Buffer (containing ethanol). The purified genomic DNA is eluted by a low salt Elution Buffer, TE or water. High quality genomic DNA can be purified in 20 minutes without phenol/chloroform extraction or alcohol precipitation, with an average DNA yield of 2 μg per buccal swab. Purified DNA, with approximately 20-30 kb, is suitable for use in PCR or other enzymatic reactions.

Specifications (Cat. # GSK100, GSK300)
  • Purify genomic DNA from buccal cell swabs in 20 minutes!
  • High Yield: up to 2 μg of pure genomic DNA per buccal swab
  • High Purity gDNA: A260/A280 = 1.8-2.0
  • DNA Size: 20-30 kb
  • Spin Columns: glass fiber membrane optimized for genomic DNA extraction
  • Elution volume: 50-100 µl
  • Storage: dry at room temperature (15-25°C)
  • PCR,
  • Restriction Endonuclease Digestions,
  • Array,
  • Southern Blots and various other enzymatic reactions
Components
  • S1 Buffer
  • S2 Buffer
  • Carrier RNA
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
  • Proteinase K
  • GD Columns
  • Filter Columns
  • 2 ml Collection Tubes
Quality Control

The quality of the Presto™ Buccal Swab gDNA Extraction Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. The purified genomic DNA (2 µg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.

Genomic DNA Extraction Kits Specifications
SpecificationsgSYNC™ DNA Extraction KitGenomic DNA Mini Kit Blood Cultured CellGenomic DNA Mini Kit TissueGenomic DNA Mini/Maxi Kit PlantPresto™ Mini gDNA Bacteria KitPresto™ Mini gDNA Yeast KitGeneius™ Micro gDNA Extraction KitPresto™ Buccal Swab gDNA Extraction Kit
Cat. #GS100
GS300
GB100
GB300
GT050
GT100
GT300
GP100
GPM010
GPM025
GBB100/101
GBB300/301
GBYB100
GBYB300
GMB100
GMB300
GSK100
GSK300
FormatSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnReagent SystemSpin ColumnSpin Column
Binding Capacity30 µg30 µg30 µg50 µg (Mini)
500 µg (Maxi)
50 µgN/A50 µg50 µg
Sample200 µl blood300 µl blood30 mg animal tissueMini: 100 mg plant tissue1x109 bacterial cells2x108 yeast cells1-100 µl bloodbuccal swabs
25 mg animal tissue200 µl buffy coatMaxi: 1 g plant tissue200 µl bloodFTA® Cards
1x107 cultured cells1x107 cultured cells1 ml urine, plasma1 ml urine
hair2x108 yeast/fungus1-100 µl saliva
MicropestleN/AN/AYesN/AN/AN/AN/AN/A
Filter ColumnN/AN/AN/AYesN/AN/AN/AYes
EnzymeProteinase KN/AProteinase KN/ALysozyme
Proteinase K
Lyticase or ZymolaseProteinase KProteinase K
Typical Yield5 µg from
200 µl blood
4-10 µg from
300 µl blood
10-40 µg from
20 mg tissue
3-5 µg from 100 mg Arabidopsis leaf (Mini)37 µg from 1x109 E. coli10-15 µg from 2x108 S. cerevisiae cells300 ng from 6 mm blood spots2 µg per swab
10-40 µg from
20 mg tissue
80 µg from 500 mg Lemon leaf (Maxi)10-15 µg from 1x109
Bacillus subtilis
Elution Volume30-100 µl30-200 µl30-200 µl30-200 µl (Mini)
1 ml (Maxi)
30-200 µl50-100 µl30-100 µl50-100 µl
Operation Time<20 minutes<25 minutes30 minutes<30 minutes<30 minutes<40 minutes<20 minutes<20 minutes
Product NameRXNSCatalogue Number
gSYNC™ DNA Extraction Kit (Tissue/Blood)100, 300GS100/300
Genomic DNA Mini Kit (Blood/Cultured Cell)100, 300GB100/300
Genomic DNA Maxi Kit (Plant)10, 25GPM010/25
Genomic DNA Maxi Kit (Blood/Cultured Cell)10, 25GDM10/25
Genomic DNA Mini Kit (Tissue)50, 100, 300GT050/100/300
Genomic DNA Mini Kit (Plant)100GP100
Geneius™ Micro gDNA Extraction Kit100GMB100/300
Presto™ Buccal Swab gDNA Extraction Kit100GSK100/300
Presto™ Mini gDNA Yeast Kit100, 300GBYB100/300
Presto™ Mini gDNA Bacteria Kit100, 300GBB100/101/300/301
Presto™ 96 Well Blood Genomic DNA Extraction Kit4 x 96, 10 x 9696GBP04/10
Presto™ 96 Well Plant Genomic DNA Extraction Kit4 x 96, 10 x 9696GPP04/10
Presto™ 96 Well gDNA Bacteria Kit4 x 96, 10 x 9696GBB04/10