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Presto™ Mini gDNA Yeast Kit

DNA Extraction/Genomic DNA Purification

The Presto™ Mini gDNA Yeast Kit was designed for rapid isolation of genomic DNA from cultured yeast and other fungus species. The yeast/fungus cells are directly added into beadbeating tubes containing ceramic beads and lysis buffer. Samples are efficiently lysed without the use of degestive enzymes (such as Lyticase or Zymolase) using a bead beating instrument or a standard vortex. The cell lysate is then mixed with Binding Buffer and the genomic DNA is bound by the GD Column. The column is then washed and the DNA is eluted with Elution Buffer. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 40 minutes. The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions

Specifications (Cat. # GBYB100, GBYB300)
  • Purify genomic DNA from (up to 2 x 108) yeast and other fungus species
  • Genomic DNA Yield: up to 10 µg of genomic DNA from 2 x 108 Saccharomyces cerevisiae
  • Format: beadbeating tubes and spin columns
  • Elution volume: 30-200 µl
  • Time: 40 minutes
  • Storage: dry at room temperature (15-25°C)
  • PCR,
  • Restricion enzyme digestion,
  • Sequencing reactions
  • GT Buffer
  • PR Buffer
  • GB Buffer
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer
  • RNase A (50 mg/m)
  • GD Columns
  • Beadbeating Tubes Type B
  • 2 ml Collection Tubes
Quality Control

The quality of the Presto™ Mini gDNA Yeast DNA Kit is tested on a lot-to-lot basis by isolating genomic DNA from 2 x108 Saccharomyces cerevisiae cells. Following the purification process, greater than 8 μg of genomic DNA is obtained and the A260/A280 ratio is between 1.7-2.0. The purified genomic DNA is analyzed by electrophoresis.

Genomic DNA Extraction Kits Specifications
SpecificationsgSYNC™ DNA Extraction KitGenomic DNA Mini Kit Blood Cultured CellGenomic DNA Mini Kit TissueGenomic DNA Mini/Maxi Kit PlantPresto™ Mini gDNA Bacteria KitPresto™ Mini gDNA Yeast KitGeneius™ Micro gDNA Extraction KitPresto™ Buccal Swab gDNA Extraction Kit
Cat. #GS100
FormatSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnReagent SystemSpin ColumnSpin Column
Binding Capacity30 µg30 µg30 µg50 µg (Mini)
500 µg (Maxi)
50 µgN/A50 µg50 µg
Sample200 µl blood300 µl blood30 mg animal tissueMini: 100 mg plant tissue1x109 bacterial cells2x108 yeast cells1-100 µl bloodbuccal swabs
25 mg animal tissue200 µl buffy coatMaxi: 1 g plant tissue200 µl bloodFTA® Cards
1x107 cultured cells1x107 cultured cells1 ml urine, plasma1 ml urine
hair2x108 yeast/fungus1-100 µl saliva
Filter ColumnN/AN/AN/AYesN/AN/AN/AYes
EnzymeProteinase KN/AProteinase KN/ALysozyme
Proteinase K
Lyticase or ZymolaseProteinase KProteinase K
Typical Yield5 µg from
200 µl blood
4-10 µg from
300 µl blood
10-40 µg from
20 mg tissue
3-5 µg from 100 mg Arabidopsis leaf (Mini)37 µg from 1x109 E. coli10-15 µg from 2x108 S. cerevisiae cells300 ng from 6 mm blood spots2 µg per swab
10-40 µg from
20 mg tissue
80 µg from 500 mg Lemon leaf (Maxi)10-15 µg from 1x109
Bacillus subtilis
Elution Volume30-100 µl30-200 µl30-200 µl30-200 µl (Mini)
1 ml (Maxi)
30-200 µl50-100 µl30-100 µl50-100 µl
Operation Time<20 minutes<25 minutes30 minutes<30 minutes<30 minutes<40 minutes<20 minutes<20 minutes
Product NameRXNSCatalogue Number
gSYNC™ DNA Extraction Kit (Tissue/Blood)100, 300GS100/300
Genomic DNA Mini Kit (Blood/Cultured Cell)100, 300GB100/300
Genomic DNA Maxi Kit (Plant)10, 25GPM010/25
Genomic DNA Maxi Kit (Blood/Cultured Cell)10, 25GDM10/25
Genomic DNA Mini Kit (Tissue)50, 100, 300GT050/100/300
Genomic DNA Mini Kit (Plant)100GP100
Geneius™ Micro gDNA Extraction Kit100GMB100/300
Presto™ Buccal Swab gDNA Extraction Kit100GSK100/300
Presto™ Mini gDNA Yeast Kit100, 300GBYB100/300
Presto™ Mini gDNA Bacteria Kit100, 300GBB100/101/300/301
Presto™ 96 Well Blood Genomic DNA Extraction Kit4 x 96, 10 x 9696GBP04/10
Presto™ 96 Well Plant Genomic DNA Extraction Kit4 x 96, 10 x 9696GPP04/10
Presto™ 96 Well gDNA Bacteria Kit4 x 96, 10 x 9696GBB04/10