Presto™ Mini RNA Bacteria Kit with DNase

RNA Extraction/Total RNA

The Presto™ Mini RNA Bacteria Kit was designed for total RNA purification from Gram (-) negative bacteria and Gram (+) positive bacteria using an efficient RNA miniprep system. This RNA Kit includes Bacteria Lysis Buffer and Lysozyme to reduce sample preparation time and minimize hands on time. The Presto™ Mini RNA Bacteria Kit uses detergents and chaotropic salt to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound by the glass fiber matrix of the RNA spin column and once any contaminants have been removed, using the Wash Buffer, the purified total RNA is eluted by RNase-free Water. High quality total RNA can be purified within 20 minutes and is ready for use in RT-PCR, Northern Blotting, Primer Extension, mRNA Selection and cDNA Synthesis. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes.

Specifications (Cat. # RBB050, RBB100, RBB300)

Purify total RNA within 20 minutes!
High Yield: up to 60 μg of pure RNA (1 x 109 Escherichia coli: 40-45 µg, 1 x 10⁹ Bacillus subtilis: 50-55 µg)
Wide Variety of Bacteria Sample Strains: Gram (+) Positive and Gram (-) Negative bacterial cells (up to 1 x 10⁹)
Convenient: Includes Bacteria Lysis Buffer and Lysozyme
Spin Columns: glass fiber membrane optimized for total RNA extraction
Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
Storage: dry at room temperature (15-25°C), Lysozyme is shipped at room temperature and should be stored at -20°C for extended periods

RT-PCR,
Northern Blotting,
Primer Extension,
mRNA Selection,
cDNA Synthesis

Components

Bacteria Lysis Buffer
Lysozyme
RB Buffer
W1 Buffer
Wash Buffer
RNase-free Water
RB Columns
2 ml Collection Tubes

Quality Control

The quality of the Presto™ Mini RNA Bacteria Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. The purified RNA from Escherichia coli cells (up to 1 x 10⁹), (A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.

Total RNA Extraction Kits Specifications

Specifications	Total RNA Mini Kit Blood Cultured Cell	Total RNA Mini Kit Tissue	Total RNA Mini Kit Plant	Presto™ Mini RNA Bacteria Kit	Presto™ Mini RNA Yeast Kit	miRNA Isolation Kit	GENEzol™ TriRNA Pure Kit
Cat. #	RB050 RB100 RB300	RT050 RT100 RT300	RP050 RP100 RP300	RBB050 RBB100 RBB300	RBY050 RBY100 RBY300	RMI050	GZX050 GZX100 GZX200
Format	Spin Column	Spin Column	Spin Column	Spin Column	Spin Column	Spin Column	Tri Reagent Spin Column
Binding Capacity	50 µg	50 µg	50 µg	50 µg	50 µg	50 µg	50 µg
Sample	blood, cells	animal tissue	plant tissue	bacterial cells	yeast cells	animal tissue, cells	blood,cells, tissue, body fluids, bacteria
Micropestle	N/A	Yes	N/A	N/A	N/A	Yes	N/A
Filter Column	N/A	Yes	Yes	N/A	N/A	N/A	N/A
Enzyme	N/A	N/A	N/A	Lysozyme	N/A	N/A	N/A
Typical Yield	2-3 µg from 300 µl blood, 25-30 µg from 106 cells	5-30 µg	5-30 µg	40-50 µg from 1x109 bacterial cells	15-20 µg from 5x107 yeast cells	5-30 µg	5-30 µg
Elution Volume	50 µl	50 µl	50 µl	50 µl	50 µl	50 µl	25-50 µl
Time	<20 min.	<15 min.	<20 min.	<20 min.	<20 min.	<30 min.	<15 min.

Presto™ Mini RNA Bacteria Kit

Product Name	RXNS	Catalogue Number
Total RNA Mini Kit (Blood/Cultured Cell)	50/100/300	RB050/100/300, RBD050/100/300
Total RNA Mini Kit (Tissue)	50/100/300	RT050/100/300, RTD050/100/300
Total RNA Mini Kit (Plant)	50/100/300	RP050/100/300, RPD050/100/300
Presto™ Mini RNA Bacteria Kit	50/100/300	RBB050/100/300, RBBD050/100/300
Presto™ Mini RNA Yeast Kit	50/100/300	RBY050/100/300, RBYD050/100/300
miRNA Isolation Kit	50	RMI050
GENEzol™ TriRNA Pure Kit	50/100/200	GZX050/100/200, GZXD050/100/200
GENEzol™ 96 Well TriRNA Pure Kit	4 x 96/10 x 96	96GZX04/10