Presto™ Mini RNA Yeast Advanced Kit
RNA Extraction/Total RNA
The Presto™ Mini RNA Yeast Advanced Kit was designed for total RNA purification from yeast and a wide variety of other fungus species using an efficient RNA miniprep system with beadbeating tubes.The Presto™ Mini RNA Yeast Advanced Kit uses detergents and chaotropic salt to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column and once any contaminants have been removed, using the Wash Buffer, the purified total RNA is eluted by RNase-free Water. High quality total RNA can be purified within 30 minutes and is ready for use in RT-PCR, Northern Blotting, Primer Extension, mRNA Selection and cDNA Synthesis. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes.
Specifications (Cat. # RBYA050, RBYA100, RBYA300)
- Purify total RNA within 20 minutes!
- High Yield: 5-30 μg of pure RNA
- Convenient: Includes Beadbeating tubes
- Sample: up to 5 x 107 yeast or other fungus species
- Spin Columns: glass fiber membrane optimized for total RNA extraction
- Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
- Storage: dry at room temperature (15-25°C)
- RT-PCR,
- Northern Blotting,
- Primer Extension,
- mRNA Selection,
- cDNA Synthesis
Components
- Beadbeating tubes
- RB Buffer, RT Buffer and PR Buffer
- W1 Buffer
- Wash Buffer
- RNase-free Water
- RB Columns
- 2 ml Collection Tubes
Quality Control
The quality of the Presto™ Mini RNA Yeast Advanced Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. The purified RNA (A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.
Total RNA Extraction Kits Specifications
| Specifications | Total RNA Mini Kit Blood Cultured Cell | Total RNA Mini Kit Tissue | Total RNA Mini Kit Plant | Presto™ Mini RNA Bacteria Kit | Presto™ Mini RNA Yeast Kit | miRNA Isolation Kit | GENEzol™ TriRNA Pure Kit | ||
| Cat. # | RB050 RB100 RB300 | RT050 RT100 RT300 | RP050 RP100 RP300 | RBB050 RBB100 RBB300 | RBY050 RBY100 RBY300 | RMI050 | GZX050 GZX100 GZX200 | ||
| Format | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Tri Reagent Spin Column | ||
| Binding Capacity | 50 µg | 50 µg | 50 µg | 50 µg | 50 µg | 50 µg | 50 µg | ||
| Sample | blood, cells | animal tissue | plant tissue | bacterial cells | yeast cells | animal tissue, cells | blood,cells, tissue, body fluids, bacteria | ||
| Micropestle | N/A | Yes | N/A | N/A | N/A | Yes | N/A | ||
| Filter Column | N/A | Yes | Yes | N/A | N/A | N/A | N/A | ||
| Enzyme | N/A | N/A | N/A | Lysozyme | N/A | N/A | N/A | ||
| Typical Yield | 2-3 µg from 300 µl blood, 25-30 µg from 106 cells | 5-30 µg | 5-30 µg | 40-50 µg from 1x109 bacterial cells | 15-20 µg from 5x107 yeast cells | 5-30 µg | 5-30 µg | ||
| Elution Volume | 50 µl | 50 µl | 50 µl | 50 µl | 50 µl | 50 µl | 25-50 µl | ||
| Time | <20 min. | <15 min. | <20 min. | <20 min. | <20 min. | <30 min. | <15 min. |
| Product Name | RXNS | Catalogue Number | ||
| Total RNA Mini Kit (Blood/Cultured Cell) | 50/100/300 | RB050/100/300, RBD050/100/300 | ||
| Total RNA Mini Kit (Tissue) | 50/100/300 | RT050/100/300, RTD050/100/300 | ||
| Total RNA Mini Kit (Plant) | 50/100/300 | RP050/100/300, RPD050/100/300 | ||
| Presto™ Mini RNA Bacteria Kit | 50/100/300 | RBB050/100/300, RBBD050/100/300 | ||
| Presto™ Mini RNA Yeast Kit | 50/100/300 | RBY050/100/300, RBYD050/100/300 | ||
| miRNA Isolation Kit | 50 | RMI050 | ||
| GENEzol™ TriRNA Pure Kit | 50/100/200 | GZX050/100/200, GZXD050/100/200 | ||
| GENEzol™ 96 Well TriRNA Pure Kit | 4 x 96/10 x 96 | 96GZX04/10 |
