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Presto™ Mini RNA Yeast Kit with DNase

RNA Extraction/Total RNA

The Presto™ Mini RNA Yeast Kit was designed for total RNA purification from yeast and a wide variety of other fungus species using an efficient RNA miniprep system. This RNA Kit includes Sorbitol Buffer to reduce sample preparation time and minimize hands on time. The Presto™ Mini RNA Yeast Kit uses detergents and chaotropic salt to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound by the glass fiber matrix of the spin column and once any contaminants have been removed, using the Wash Buffer, the purified total RNA is eluted by RNase-free Water. High quality total RNA can be purified within 20 minutes and is ready for use in RT-PCR, Northern Blotting, Primer Extension, mRNA Selection and cDNA Synthesis. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes and DNase I for DNA sensitive downstream applications.

Specifications (Cat. # RBYD050, RBYD100, RBYD300)
  • Purify total RNA within 20 minutes!
  • High Yield: 5-30 μg of pure RNA
  • Convenient: Includes premixed Sorbitol Buffer
  • Sample: up to 5 x 107 yeast or other fungus species
  • Spin Columns: glass fiber membrane optimized for total RNA extraction
  • Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
  • Storage: dry at room temperature (15-25°C), DNase I is shipped at room temperature and should be stored at -20°C for extended periods
  • RT-PCR,
  • Northern Blotting,
  • Primer Extension,
  • mRNA Selection,
  • cDNA Synthesis
Components
  • Sorbitol Buffer
  • RB Buffer
  • DNase I
  • DNase I Reaction Buffer
  • W1 Buffer
  • Wash Buffer
  • RNase-free Water
  • RB Columns
  • 2 ml Collection Tubes
Quality Control

The quality of the Presto™ Mini RNA Yeast Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. The purified RNA (A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.

Total RNA Extraction Kits Specifications
SpecificationsTotal RNA Mini Kit Blood Cultured CellTotal RNA Mini Kit TissueTotal RNA Mini Kit PlantPresto™ Mini RNA Bacteria KitPresto™ Mini RNA Yeast KitmiRNA Isolation KitGENEzol™ TriRNA Pure Kit
Cat. #RB050
RB100
RB300
RT050
RT100
RT300
RP050
RP100
RP300
RBB050
RBB100
RBB300
RBY050
RBY100
RBY300
RMI050GZX050
GZX100
GZX200
FormatSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnTri Reagent Spin Column
Binding Capacity50 µg50 µg50 µg50 µg50 µg50 µg50 µg
Sampleblood, cellsanimal tissueplant tissuebacterial cellsyeast cellsanimal tissue, cellsblood,cells,
tissue, body fluids, bacteria
MicropestleN/AYesN/AN/AN/AYesN/A
Filter ColumnN/AYesYesN/AN/AN/AN/A
EnzymeN/AN/AN/ALysozymeN/AN/AN/A
Typical Yield2-3 µg from 300 µl blood, 25-30 µg from 106 cells5-30 µg5-30 µg40-50 µg from 1x109 bacterial cells15-20 µg from 5x107 yeast cells5-30 µg5-30 µg
Elution Volume50 µl50 µl50 µl50 µl50 µl50 µl25-50 µl
Time<20 min.<15 min.<20 min.<20 min.<20 min.<30 min.<15 min.
Product NameRXNSCatalogue Number
Total RNA Mini Kit (Blood/Cultured Cell)50/100/300RB050/100/300, RBD050/100/300
Total RNA Mini Kit (Tissue)50/100/300RT050/100/300, RTD050/100/300
Total RNA Mini Kit (Plant)50/100/300RP050/100/300, RPD050/100/300
Presto™ Mini RNA Bacteria Kit50/100/300RBB050/100/300, RBBD050/100/300
Presto™ Mini RNA Yeast Kit50/100/300RBY050/100/300, RBYD050/100/300
miRNA Isolation Kit50RMI050
GENEzol™ TriRNA Pure Kit50/100/200GZX050/100/200, GZXD050/100/200
GENEzol™ 96 Well TriRNA Pure Kit4 x 96/10 x 9696GZX04/10