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Presto™ miRNA Purification Kit

RNA Extraction/Total RNA

The Presto™ miRNA Purification Kit is designed for purifying and enriching of small RNA molecules (< 200 nt) from a wide variety of samples, such as animal tissues, cultured cells and bacterial cells. These small RNAs include microRNA (miRNA), short interfering RNA (siRNA), as well as tRNA and 5S ribosomal RNA. miRNAs and siRNAs are typically 20-25 nucleotides long and vital for regulating gene expression by binding to mRNA molecules and affecting their stability or translation. Purification is based on spin column technology without the use of phenol and chloroform. Briefly, the cells or tissues are lysed using detergents and chaotropic salt. The larger RNA fragments in the chaotropic salt are bound by the glass fiber matrix of the first spin column while the smaller RNA molecules pass through into the flow-through. The smaller RNA molecules in the flow-through are bound by the second spin column once any contaminants have been removed using the Wash Buffer. The small RNA molecules are then eluted using the RNase-free water, and are ready to be used in various applications, including RT-PCR, northern blotting and microarray analysis.

Specifications (Cat. # PMI004, PMI050, PMI100)
  • Purify small RNA molecules (< 200 nt) within 30 minutes!
  • Spin Columns: glass fiber membrane optimized for micro RNA extraction
  • No phenol or chloroform extractions
  • Minimal contamination from large RNA fragments
  • Large RNA fragments can be separately isolated during the extraction procedure
  • High quality small RNA can be used in various downstream applications
  • Storage: dry at room temperature (15-25°C)
  • RT-PCR,
  • Northern Blotting,
  • Microarray analysis
  • PMI Buffer
  • W1 Buffer
  • Wash Buffer
  • RNase-free Water
  • RB Columns
  • 2 ml Collection Tubes
Quality Control

The quality of the  Presto™ miRNA Purification Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. Small RNAs from 1 x 106 Hela cells  are extracted using the Presto™ miRNA Purification Kit. The purified small RNA  is analyzed using capillary electrophoresis.

Total RNA Extraction Kits Specifications
SpecificationsTotal RNA Mini Kit Blood Cultured CellTotal RNA Mini Kit TissueTotal RNA Mini Kit PlantPresto™ Mini RNA Bacteria KitPresto™ Mini RNA Yeast KitmiRNA Isolation KitGENEzol™ TriRNA Pure Kit
Cat. #RB050
FormatSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnTri Reagent Spin Column
Binding Capacity50 µg50 µg50 µg50 µg50 µg50 µg50 µg
Sampleblood, cellsanimal tissueplant tissuebacterial cellsyeast cellsanimal tissue, cellsblood,cells,
tissue, body fluids, bacteria
Filter ColumnN/AYesYesN/AN/AN/AN/A
Typical Yield2-3 µg from 300 µl blood, 25-30 µg from 106 cells5-30 µg5-30 µg40-50 µg from 1x109 bacterial cells15-20 µg from 5x107 yeast cells5-30 µg5-30 µg
Elution Volume50 µl50 µl50 µl50 µl50 µl50 µl25-50 µl
Time<20 min.<15 min.<20 min.<20 min.<20 min.<30 min.<15 min.
Product NameRXNSCatalogue Number
Total RNA Mini Kit (Blood/Cultured Cell)50/100/300RB050/100/300, RBD050/100/300
Total RNA Mini Kit (Tissue)50/100/300RT050/100/300, RTD050/100/300
Total RNA Mini Kit (Plant)50/100/300RP050/100/300, RPD050/100/300
Presto™ Mini RNA Bacteria Kit50/100/300RBB050/100/300, RBBD050/100/300
Presto™ Mini RNA Yeast Kit50/100/300RBY050/100/300, RBYD050/100/300
miRNA Isolation Kit50RMI050
GENEzol™ TriRNA Pure Kit50/100/200GZX050/100/200, GZXD050/100/200
GENEzol™ 96 Well TriRNA Pure Kit4 x 96/10 x 9696GZX04/10