Presto™ Soil DNA Extraction Kit
DNA Extraction/Genomic DNA Purification
The Presto™ Soil DNA Extraction Kit was designed for rapid isolation of genomic DNA from microorganisms such as bacteria, archaea, fungi, and algae in soil samples. The soil sample is homogenized using SL1 Lysis Buffer combined with ceramic beads in Beadbeating Tubes. Insoluble particles, proteins and PCR inhibitors such as humic acid are then precipitated with a unique inhibitor removal buffer (SL2). In addition, residual PCR inhibitors remaining in the clear supernatant are further removed by passing through a specialized PCR Inhibitor Removal Column. The flow-through is then mixed with a binding buffer (SL3) and the genomic DNA is bound by the GD Column. The column is then washed and the DNA is eluted with Elution Buffer. DNA phenol extraction or alcohol precipitation is not required and the entire soil DNA extraction procedure can be completed within 40 minutes. The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions.
Specifications (Cat. # SLD050, SLD100)
- Purify genomic DNA from soil within 40 minutes!
- Sample: 250-500 mg of soil
- High Yield: up to 5 µg
- High Quality DNA: A260/A280 = 1.7-2.0
- Spin Columns: glass fiber membrane optimized for genomic DNA extraction
- Elution Volume: 30-100 µl
- Storage: dry at room temperature (15-25°C)
- PCR,
- AFLP/PADP,
- RFLP,
- Southern Blotting,
- Real-time PCR
Components
- SL1 Buffer
- SL2 Buffer
- SL3 Buffer
- Wash Buffer
- Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
- Inhibitor Removal Columns
- GD Columns
- Beadbeating Tubes Type C
- 2 ml Centrifuge Tubes
- 2 ml Collection Tubes
Quality Control
The quality of the Presto™ Soil DNA Extraction Kit is tested on a lot-to-lot basis by isolating genomic DNA from 250 mg soil samples. Following the purification process, a yield of more than 2 μg of genomic DNA is obtained and the A260/A280 ratio is between 1.7-2.0. The purified genomic DNA is analyzed by electrophoresis.
Genomic DNA Extraction Kit Specifications
| Specifications | gSYNC™ DNA Extraction Kit | Genomic DNA Mini Kit Blood Cultured Cell | Genomic DNA Mini Kit Tissue | Genomic DNA Mini/Maxi Kit Plant | Presto™ Mini gDNA Bacteria Kit | Presto™ Mini gDNA Yeast Kit | Geneius™ Micro gDNA Extraction Kit | Presto™ Buccal Swab gDNA Extraction Kit | ||
| Cat. # | GS100 GS300 | GB100 GB300 | GT050 GT100 GT300 | GP100 GPM010 GPM025 | GBB100/101 GBB300/301 | GBYB100 GBYB300 | GMB100 GMB300 | GSK100 GSK300 | ||
| Format | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Reagent System | Spin Column | Spin Column | ||
| Binding Capacity | 30 µg | 30 µg | 30 µg | 50 µg (Mini) 500 µg (Maxi) | 50 µg | N/A | 50 µg | 50 µg | ||
| Sample | 200 µl blood | 300 µl blood | 30 mg animal tissue | Mini: 100 mg plant tissue | 1x109 bacterial cells | 2x108 yeast cells | 1-100 µl blood | buccal swabs | ||
| 25 mg animal tissue | 200 µl buffy coat | Maxi: 1 g plant tissue | 200 µl blood | FTA® Cards | ||||||
| 1x107 cultured cells | 1x107 cultured cells | 1 ml urine, plasma | 1 ml urine | |||||||
| hair | 2x108 yeast/fungus | 1-100 µl saliva | ||||||||
| Micropestle | N/A | N/A | Yes | N/A | N/A | N/A | N/A | N/A | ||
| Filter Column | N/A | N/A | N/A | Yes | N/A | N/A | N/A | Yes | ||
| Enzyme | Proteinase K | N/A | Proteinase K | N/A | Lysozyme Proteinase K | Lyticase or Zymolase | Proteinase K | Proteinase K | ||
| Typical Yield | 5 µg from 200 µl blood | 4-10 µg from 300 µl blood | 10-40 µg from 20 mg tissue | 3-5 µg from 100 mg Arabidopsis leaf (Mini) | 37 µg from 1x109 E. coli | 10-15 µg from 2x108 S. cerevisiae cells | 300 ng from 6 mm blood spots | 2 µg per swab | ||
| 10-40 µg from 20 mg tissue | 80 µg from 500 mg Lemon leaf (Maxi) | 10-15 µg from 1x109 Bacillus subtilis | ||||||||
| Elution Volume | 30-100 µl | 30-200 µl | 30-200 µl | 30-200 µl (Mini) 1 ml (Maxi) | 30-200 µl | 50-100 µl | 30-100 µl | 50-100 µl | ||
| Operation Time | <20 minutes | <25 minutes | 30 minutes | <30 minutes | <30 minutes | <40 minutes | <20 minutes | <20 minutes |
| Product Name | RXNS | Catalogue Number | ||
| gSYNC™ DNA Extraction Kit (Tissue/Blood) | 100, 300 | GS100/300 | ||
| Genomic DNA Mini Kit (Blood/Cultured Cell) | 100, 300 | GB100/300 | ||
| Genomic DNA Maxi Kit (Plant) | 10, 25 | GPM010/25 | ||
| Genomic DNA Maxi Kit (Blood/Cultured Cell) | 10, 25 | GDM10/25 | ||
| Genomic DNA Mini Kit (Tissue) | 50, 100, 300 | GT050/100/300 | ||
| Genomic DNA Mini Kit (Plant) | 100 | GP100 | ||
| Geneius™ Micro gDNA Extraction Kit | 100 | GMB100/300 | ||
| Presto™ Buccal Swab gDNA Extraction Kit | 100 | GSK100/300 | ||
| Presto™ Mini gDNA Yeast Kit | 100, 300 | GBYB100/300 | ||
| Presto™ Mini gDNA Bacteria Kit | 100, 300 | GBB100/101/300/301 | ||
| Presto™ 96 Well Blood Genomic DNA Extraction Kit | 4 x 96, 10 x 96 | 96GBP04/10 | ||
| Presto™ 96 Well Plant Genomic DNA Extraction Kit | 4 x 96, 10 x 96 | 96GPP04/10 | ||
| Presto™ 96 Well gDNA Bacteria Kit | 4 x 96, 10 x 96 | 96GBB04/10 |
