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Presto™ Stool gDNA Extraction Kit

DNA Extraction/Genomic DNA Purification

The Presto™ Stool gDNA Extraction Kit is designed for rapid isolation of genomic DNA from microorganisms, such as bacteria and fungi in stool samples. The stool sample is homogenized and disrupted using a lysis buffer and ceramic beads. Insoluble particles, proteins and PCR inhibitors such as humic acid are then precipitated with a proprietary inhibitor removal buffer. Residual inhibitors are further removed by passing through a specialized PCR inhibitor removal column. Genomic DNA in the sample is then bound by the GD Column followed by wash and elution. DNA phenol extraction or alcohol precipitation is not required and the entire procedure can be completed within 50 minutes. The purified genomic DNA is ready for use in PCR, restriction enzyme digestion, and sequencing reactions.

Specifications (Cat. # STLD050, STLD100)
  • Purify genomic DNA from stool samples within 50 minutes!
  • Sample: 180-200 mg of fresh or frozen stool samples
  • Binding Capacity: 50 µg
  • High Quality DNA: A260/A280 = 1.8-2.0
  • Format: beadbeating tubes, inhibitor removal columns and spin columns
  • Elution Volume: 30-100 µl
  • Storage: dry at room temperature (15-25°C)
  • PCR,
  • AFLP/PADP,
  • RFLP,
  • Southern Blotting,
  • Real-time PCR
Components
  • ST1 Buffer
  • ST2 Buffer
  • ST3 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25ºC)
  • Inhibitor Removal Columns
  • GD Columns
  • Beadbeating Tubes Type C
  • 2 ml Centrifuge Tubes
  • 2 ml Collection Tubes
Quality Control

The quality of the Presto™ Stool DNA Extraction Kit is tested on a lot-to-lot basis by isolating genomic DNA from 200 mg stool samples. Following the purification process, the purified genomic DNA (A260/A280 ratio between 1.8-2.0) is analyzed by electrophoresis.

Genomic DNA Extraction Kits Specifications
SpecificationsgSYNC™ DNA Extraction KitGenomic DNA Mini Kit Blood Cultured CellGenomic DNA Mini Kit TissueGenomic DNA Mini/Maxi Kit PlantPresto™ Mini gDNA Bacteria KitPresto™ Mini gDNA Yeast KitGeneius™ Micro gDNA Extraction KitPresto™ Buccal Swab gDNA Extraction Kit
Cat. #GS100
GS300
GB100
GB300
GT050
GT100
GT300
GP100
GPM010
GPM025
GBB100/101
GBB300/301
GBYB100
GBYB300
GMB100
GMB300
GSK100
GSK300
FormatSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnReagent SystemSpin ColumnSpin Column
Binding Capacity30 µg30 µg30 µg50 µg (Mini)
500 µg (Maxi)
50 µgN/A50 µg50 µg
Sample200 µl blood300 µl blood30 mg animal tissueMini: 100 mg plant tissue1x109 bacterial cells2x108 yeast cells1-100 µl bloodbuccal swabs
25 mg animal tissue200 µl buffy coatMaxi: 1 g plant tissue200 µl bloodFTA® Cards
1x107 cultured cells1x107 cultured cells1 ml urine, plasma1 ml urine
hair2x108 yeast/fungus1-100 µl saliva
MicropestleN/AN/AYesN/AN/AN/AN/AN/A
Filter ColumnN/AN/AN/AYesN/AN/AN/AYes
EnzymeProteinase KN/AProteinase KN/ALysozyme
Proteinase K
Lyticase or ZymolaseProteinase KProteinase K
Typical Yield5 µg from
200 µl blood
4-10 µg from
300 µl blood
10-40 µg from
20 mg tissue
3-5 µg from 100 mg Arabidopsis leaf (Mini)37 µg from 1x109 E. coli10-15 µg from 2x108 S. cerevisiae cells300 ng from 6 mm blood spots2 µg per swab
10-40 µg from
20 mg tissue
80 µg from 500 mg Lemon leaf (Maxi)10-15 µg from 1x109
Bacillus subtilis
Elution Volume30-100 µl30-200 µl30-200 µl30-200 µl (Mini)
1 ml (Maxi)
30-200 µl50-100 µl30-100 µl50-100 µl
Operation Time<20 minutes<25 minutes30 minutes<30 minutes<30 minutes<40 minutes<20 minutes<20 minutes
Product NameRXNSCatalogue Number
gSYNC™ DNA Extraction Kit (Tissue/Blood)100, 300GS100/300
Genomic DNA Mini Kit (Blood/Cultured Cell)100, 300GB100/300
Genomic DNA Maxi Kit (Plant)10, 25GPM010/25
Genomic DNA Maxi Kit (Blood/Cultured Cell)10, 25GDM10/25
Genomic DNA Mini Kit (Tissue)50, 100, 300GT050/100/300
Genomic DNA Mini Kit (Plant)100GP100
Geneius™ Micro gDNA Extraction Kit100GMB100/300
Presto™ Buccal Swab gDNA Extraction Kit100GSK100/300
Presto™ Mini gDNA Yeast Kit100, 300GBYB100/300
Presto™ Mini gDNA Bacteria Kit100, 300GBB100/101/300/301
Presto™ 96 Well Blood Genomic DNA Extraction Kit4 x 96, 10 x 9696GBP04/10
Presto™ 96 Well Plant Genomic DNA Extraction Kit4 x 96, 10 x 9696GPP04/10
Presto™ 96 Well gDNA Bacteria Kit4 x 96, 10 x 9696GBB04/10