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Small DNA Fragments Extraction Kit

DNA Extraction/Gel Extraction

The Small DNA Fragments Extraction Kit is a Gel Extraction Kit and PCR Cleanup Kit in 1 convenient system optimized to recover or concentrate 40-200 bp DNA fragments from agarose gel, PCR, or other enzymatic reactions. For processing 70 bp-10 kb DNA fragments, the Gel/PCR DNA Fragments Extraction Kit is also available. For processing 10-50 kb DNA fragments, the Large DNA Fragments Extraction Kit is also available. Chaotropic salt is used to dissolve agarose gel and denature enzymes. DNA fragments in the chaotropic salt are bound by the glass fiber matrix of the spin column. Contaminants are removed with a Wash Buffer (containing ethanol) and the purified DNA fragments are eluted by a low salt Elution Buffer, TE or water. Salts, enzymes and unincorporated nucleotides can be effectively removed from the reaction mixture without phenol extraction or alcohol precipitation. Typically, recoveries are up to 90% for Gel Extraction and up to 95% for PCR Cleanup. The eluted DNA is ready for use in PCR, Fluorescent or Radioactive Sequencing, Restriction Enzyme Digestion, DNA Labeling and Ligation.

Specifications (Cat. # DF101, DF301)
  • Purify small DNA in as little as 10 minutes!
  • Up to 95% recovery of small DNA fragments
  • Optimized for 40 bp–200 bp DNA fragment recovery
  • Sample Size: up to 300 mg (agarose gel slice), up to 100 μl (PCR products)
  • Spin Columns: glass fiber membrane optimized for both gel extraction and PCR cleanup of small DNA fragments
  • Elution Volume: 20-50 µl
  • Storage: dry at room temperature (15-25ºC)
  • Fluorescent and Radioactive Sequencing,
  • PCR,
  • Restriction Enzyme Digestion,
  • DNA Labeling,
  • Ligation
Components
  • SD Buffer
  • W1 Buffer
  • Wash Buffer
  • Elution Buffer (10 mM Tris-HCl, pH8.5 at 25°C)
  • SD Columns
  • 2 ml Collection Tubes
Quality Control

The quality of the Small DNA Fragments Extraction Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. DNA fragments are purified from either agarose gel, PCR products or other aqueous solutions and analyzed by electrophoresis.

Small DNA Fragments Extraction Kit Interactive Protocol

Gel Extraction and PCR Cleanup Kits Specifications
SpecificationsGenepHlow™ Gel Extraction KitGenepHlow™ PCR Cleanup KitGenepHlow™ Gel/PCR KitGenepHlow™ DNA Cleanup Maxi KitPresto™ 96 Well PCR Cleanup KitSmall DNA Fragments Extraction KitPresto™ Max Gel/PCR Kit
Cat. #DFG100
DFG300
DFC100
DFC300
DFH100
DFH300
DFM010
DFM025
96DFH04
96DFH10
DF101
DF301
DFL100
DFL300
FormatSpin ColumnSpin ColumnSpin ColumnMaxi Spin ColumnSpin ColumnSpin ColumnSilica Matrix
Binding Capacity10 µg10 µg10 µg200 µg10 µg10 µg5 µg
SampleAgarose Gel SlicePCR ProductAgarose Gel Slice/PCR ProductAgarose Gel Slice/Restriction Enzyme Digest ProductPCR ProductAgarose Gel Slice/PCR ProductAgarose Gel Slice/PCR Product
Fragment Size70 bp-20 kb70 bp-20 kb70 bp-20 kb100 bp-10 kb70 bp-20 kb40 bp-200 bp100 bp-50 kb
pH IndicatorYesYesYesYesN/AN/AYes
RecoveryUp to 90%Up to 95%Up to 95%Up to 80%Up to 95%Up to 95%Up to 95% (<10 kb)
Up to 80% (10-50 kb)
Elution Volume20-50 µl20-50 µl20-50 µl500-1000 µl60-80 µl20-50 µl10-20 µl
Operation Time<20 minutes<10 minutes<20 minutes<25 minutes<20 minutes<20 minutes<20 minutes
Product NameRXNSCatalogue Number
GenepHlow™ Gel Extraction Kit100/300DFG100/300
GenepHlow™ PCR Cleanup Kit100/300DFC100/300
GenepHlow™ Gel/PCR Kit100/300DFH100/300
GenepHlow™ DNA Cleanup Maxi Kit10/25DFM010/25
Presto™ 96 Well PCR Cleanup Kit4 x 96/10 x 9696DFH04/10
Small DNA Fragments Extraction Kit100/300DF101/301
Presto™ Max Gel/PCR Kit100/300DFL100/300
Gel/PCR DNA Fragments Extraction Kit100/300DF100/300
Presto™ 96 Well Gel Extraction Kit4 x 96/10 x 9696DFG04/10