Total RNA Mini Kit (Blood/Cultured Cell) with DNase
RNA Extraction/Total RNA
The RNA Kit (Blood/Cultured Cell) is a RNA Isolation Kit designed for total RNA purification from fresh whole human blood and cultured cells. This RNA Kit will purify RNA from blood within 20 minutes using an efficient RNA miniprep system. Detergents and chaotropic salt are used to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound by the glass fiber matrix of the RNA spin column and once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water and ready for use in various downstream applications. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes and DNase I for DNA sensitive downstream applications.
Specifications (Cat. # RBD050, RBD100, RBD300)
- Purify total RNA within 20 minutes!
- High Yield: up to 2 µg from whole blood, up to 30 µg from 1×106 293T cells
- Sample Volume: up to 300 µl of whole blood, cultured cells (up to 5 x 106)
- Spin Columns: glass fiber membrane optimized for total RNA extraction
- Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
- Storage: dry at room temperature (15-25°C), DNase I is shipped at room temperature and should be stored at -20°C
- RT-PCR,
- Northern Blotting,
- Primer Extension,
- mRNA Selection,
- cDNA Synthesis
Components
- RBC Lysis Buffer
- RB Buffer
- DNase I
- DNase I Reaction Buffer
- W1 Buffer
- Wash Buffer
- RNase-free Water
- RB Columns
- 2 ml Collection Tubes
Quality Control
The quality of the Total RNA Mini Kit (Blood/Cultured Cell) is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. Total RNA from 300 μl of fresh whole human blood is extracted using the Total RNA Mini Kit (Blood/Cultured Cell). The purified RNA (2 μg with an A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.
Total RNA Extraction Kits Specifications
| Specifications | Total RNA Mini Kit Blood Cultured Cell | Total RNA Mini Kit Tissue | Total RNA Mini Kit Plant | Presto™ Mini RNA Bacteria Kit | Presto™ Mini RNA Yeast Kit | miRNA Isolation Kit | GENEzol™ TriRNA Pure Kit | ||
| Cat. # | RB050 RB100 RB300 | RT050 RT100 RT300 | RP050 RP100 RP300 | RBB050 RBB100 RBB300 | RBY050 RBY100 RBY300 | RMI050 | GZX050 GZX100 GZX200 | ||
| Format | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Tri Reagent Spin Column | ||
| Binding Capacity | 50 µg | 50 µg | 50 µg | 50 µg | 50 µg | 50 µg | 50 µg | ||
| Sample | blood, cells | animal tissue | plant tissue | bacterial cells | yeast cells | animal tissue, cells | blood,cells, tissue, body fluids, bacteria | ||
| Micropestle | N/A | Yes | N/A | N/A | N/A | Yes | N/A | ||
| Filter Column | N/A | Yes | Yes | N/A | N/A | N/A | N/A | ||
| Enzyme | N/A | N/A | N/A | Lysozyme | N/A | N/A | N/A | ||
| Typical Yield | 2-3 µg from 300 µl blood, 25-30 µg from 106 cells | 5-30 µg | 5-30 µg | 40-50 µg from 1x109 bacterial cells | 15-20 µg from 5x107 yeast cells | 5-30 µg | 5-30 µg | ||
| Elution Volume | 50 µl | 50 µl | 50 µl | 50 µl | 50 µl | 50 µl | 25-50 µl | ||
| Time | <20 min. | <15 min. | <20 min. | <20 min. | <20 min. | <30 min. | <15 min. |
| Product Name | RXNS | Catalogue Number | ||
| Total RNA Mini Kit (Blood/Cultured Cell) | 50/100/300 | RB050/100/300, RBD050/100/300 | ||
| Total RNA Mini Kit (Tissue) | 50/100/300 | RT050/100/300, RTD050/100/300 | ||
| Total RNA Mini Kit (Plant) | 50/100/300 | RP050/100/300, RPD050/100/300 | ||
| Presto™ Mini RNA Bacteria Kit | 50/100/300 | RBB050/100/300, RBBD050/100/300 | ||
| Presto™ Mini RNA Yeast Kit | 50/100/300 | RBY050/100/300, RBYD050/100/300 | ||
| miRNA Isolation Kit | 50 | RMI050 | ||
| GENEzol™ TriRNA Pure Kit | 50/100/200 | GZX050/100/200, GZXD050/100/200 | ||
| GENEzol™ 96 Well TriRNA Pure Kit | 4 x 96/10 x 96 | 96GZX04/10 |
