Total RNA Mini Kit (Tissue) with DNase
RNA Extraction/Total RNA
The Total RNA Mini Kit (Tissue) is a RNA Isolation Kit designed for total RNA purification from a variety of animal tissue using an efficient RNA miniprep system. This RNA Kit will purify RNA from tissue samples within 15 minutes. Tissue samples can be efficiently homogenized in a microcentrifuge tube using the provided DNase and RNase-free Micropestle. The Total RNA Mini Kit (Tissue) uses detergents and chaotropic salt to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound by the glass fiber matrix of the RNA spin column. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water. Phenol extraction or alcohol precipitation is not required and the purified RNA is ready for use in a variety of downstream applications. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes and DNase I for DNA sensitive downstream applications.
Specifications (Cat. # RTD050, RTD100, RTD300)
- Purify total RNA within 15 minutes!
- Sample: up to 25 mg of tissue (tailsnips, liver, kidney, brain, spleen, earpunches etc.)
- High Yield: 5-30 μg of pure RNA
- RNase and DNase-free Micropestles included for simple tissue homogenization within the tube to reduce the risk of sample contamination
- Spin Columns: glass fiber membrane optimized for total RNA extraction
- Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
- Storage: dry at room temperature (15-25°C), DNase I is shipped at room temperature and should be stored at -20°C
- RT-PCR,
- Northern Blotting,
- Primer Extension,
- mRNA Selection,
- cDNA Synthesis,
- RNase Protection Assay
Components
- RB Buffer
- DNase I
- DNase I Reaction Buffer
- W1 Buffer
- Wash Buffer
- RNase-free Water
- RB Columns
- Filter Columns
- 2 ml Collection Tubes
- Micropestles
Quality Control
The quality of the Total RNA Mini Kit (Tissue) is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. Total RNA from a 25 mg fresh tissue sample is extracted using the Total RNA Mini Kit (Tissue). The purified RNA (A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.
Total RNA Extraction Kits Specifications
| Specifications | Total RNA Mini Kit Blood Cultured Cell | Total RNA Mini Kit Tissue | Total RNA Mini Kit Plant | Presto™ Mini RNA Bacteria Kit | Presto™ Mini RNA Yeast Kit | miRNA Isolation Kit | GENEzol™ TriRNA Pure Kit | ||
| Cat. # | RB050 RB100 RB300 | RT050 RT100 RT300 | RP050 RP100 RP300 | RBB050 RBB100 RBB300 | RBY050 RBY100 RBY300 | RMI050 | GZX050 GZX100 GZX200 | ||
| Format | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Spin Column | Tri Reagent Spin Column | ||
| Binding Capacity | 50 µg | 50 µg | 50 µg | 50 µg | 50 µg | 50 µg | 50 µg | ||
| Sample | blood, cells | animal tissue | plant tissue | bacterial cells | yeast cells | animal tissue, cells | blood,cells, tissue, body fluids, bacteria | ||
| Micropestle | N/A | Yes | N/A | N/A | N/A | Yes | N/A | ||
| Filter Column | N/A | Yes | Yes | N/A | N/A | N/A | N/A | ||
| Enzyme | N/A | N/A | N/A | Lysozyme | N/A | N/A | N/A | ||
| Typical Yield | 2-3 µg from 300 µl blood, 25-30 µg from 106 cells | 5-30 µg | 5-30 µg | 40-50 µg from 1x109 bacterial cells | 15-20 µg from 5x107 yeast cells | 5-30 µg | 5-30 µg | ||
| Elution Volume | 50 µl | 50 µl | 50 µl | 50 µl | 50 µl | 50 µl | 25-50 µl | ||
| Time | <20 min. | <15 min. | <20 min. | <20 min. | <20 min. | <30 min. | <15 min. |
| Product Name | RXNS | Catalogue Number | ||
| Total RNA Mini Kit (Blood/Cultured Cell) | 50/100/300 | RB050/100/300, RBD050/100/300 | ||
| Total RNA Mini Kit (Tissue) | 50/100/300 | RT050/100/300, RTD050/100/300 | ||
| Total RNA Mini Kit (Plant) | 50/100/300 | RP050/100/300, RPD050/100/300 | ||
| Presto™ Mini RNA Bacteria Kit | 50/100/300 | RBB050/100/300, RBBD050/100/300 | ||
| Presto™ Mini RNA Yeast Kit | 50/100/300 | RBY050/100/300, RBYD050/100/300 | ||
| miRNA Isolation Kit | 50 | RMI050 | ||
| GENEzol™ TriRNA Pure Kit | 50/100/200 | GZX050/100/200, GZXD050/100/200 | ||
| GENEzol™ 96 Well TriRNA Pure Kit | 4 x 96/10 x 96 | 96GZX04/10 |
