Çalışma Saatleri : 09:00 - 18:00

Total RNA Mini Kit (Tissue) with DNase

RNA Extraction/Total RNA

The Total RNA Mini Kit (Tissue) is a RNA Isolation Kit designed for total RNA purification from a variety of animal tissue using an efficient RNA miniprep system. This RNA Kit will purify RNA from tissue samples within 15 minutes. Tissue samples can be efficiently homogenized in a microcentrifuge tube using the provided DNase and RNase-free Micropestle. The Total RNA Mini Kit (Tissue) uses detergents and chaotropic salt to lyse cells and inactivate RNase. RNA in the chaotropic salt is bound by the glass fiber matrix of the RNA spin column. Once any contaminants have been removed, using the Wash Buffer (containing ethanol), the purified total RNA is eluted by RNase-free Water. Phenol extraction or alcohol precipitation is not required and the purified RNA is ready for use in a variety of downstream applications. Each Geneaid RNA Kit includes individually packaged certified DNase and RNase-free spin columns and collection tubes and DNase I for DNA sensitive downstream applications.

Specifications (Cat. # RTD050, RTD100, RTD300)
  • Purify total RNA within 15 minutes!
  • Sample: up to 25 mg of tissue (tailsnips, liver, kidney, brain, spleen, earpunches etc.)
  • High Yield: 5-30 μg of pure RNA
  • RNase and DNase-free Micropestles included for simple tissue homogenization within the tube to reduce the risk of sample contamination
  • Spin Columns: glass fiber membrane optimized for total RNA extraction
  • Individually packaged RNA spin columns and collection tubes, certified RNase and DNase-free
  • Storage: dry at room temperature (15-25°C), DNase I is shipped at room temperature and should be stored at -20°C
  • RT-PCR,
  • Northern Blotting,
  • Primer Extension,
  • mRNA Selection,
  • cDNA Synthesis,
  • RNase Protection Assay
Components
  • RB Buffer
  • DNase I
  • DNase I Reaction Buffer
  • W1 Buffer
  • Wash Buffer
  • RNase-free Water
  • RB Columns
  • Filter Columns
  • 2 ml Collection Tubes
  • Micropestles
Quality Control

The quality of the Total RNA Mini Kit (Tissue) is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. Total RNA from a 25 mg fresh tissue sample is extracted using the Total RNA Mini Kit (Tissue). The purified RNA (A260/A280 ratio of 1.8-2.0) is quantified with a spectrophotometer and checked by electrophoresis.

Total RNA Extraction Kits Specifications
SpecificationsTotal RNA Mini Kit Blood Cultured CellTotal RNA Mini Kit TissueTotal RNA Mini Kit PlantPresto™ Mini RNA Bacteria KitPresto™ Mini RNA Yeast KitmiRNA Isolation KitGENEzol™ TriRNA Pure Kit
Cat. #RB050
RB100
RB300
RT050
RT100
RT300
RP050
RP100
RP300
RBB050
RBB100
RBB300
RBY050
RBY100
RBY300
RMI050GZX050
GZX100
GZX200
FormatSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnSpin ColumnTri Reagent Spin Column
Binding Capacity50 µg50 µg50 µg50 µg50 µg50 µg50 µg
Sampleblood, cellsanimal tissueplant tissuebacterial cellsyeast cellsanimal tissue, cellsblood,cells,
tissue, body fluids, bacteria
MicropestleN/AYesN/AN/AN/AYesN/A
Filter ColumnN/AYesYesN/AN/AN/AN/A
EnzymeN/AN/AN/ALysozymeN/AN/AN/A
Typical Yield2-3 µg from 300 µl blood, 25-30 µg from 106 cells5-30 µg5-30 µg40-50 µg from 1x109 bacterial cells15-20 µg from 5x107 yeast cells5-30 µg5-30 µg
Elution Volume50 µl50 µl50 µl50 µl50 µl50 µl25-50 µl
Time<20 min.<15 min.<20 min.<20 min.<20 min.<30 min.<15 min.
Product NameRXNSCatalogue Number
Total RNA Mini Kit (Blood/Cultured Cell)50/100/300RB050/100/300, RBD050/100/300
Total RNA Mini Kit (Tissue)50/100/300RT050/100/300, RTD050/100/300
Total RNA Mini Kit (Plant)50/100/300RP050/100/300, RPD050/100/300
Presto™ Mini RNA Bacteria Kit50/100/300RBB050/100/300, RBBD050/100/300
Presto™ Mini RNA Yeast Kit50/100/300RBY050/100/300, RBYD050/100/300
miRNA Isolation Kit50RMI050
GENEzol™ TriRNA Pure Kit50/100/200GZX050/100/200, GZXD050/100/200
GENEzol™ 96 Well TriRNA Pure Kit4 x 96/10 x 9696GZX04/10