TriRNA Pure Kit with DNase
RNA Extraction/Tri RNA
The TriRNA Pure Kit is a spin column system for purification of high-quality total RNA from a variety of samples. Initially, samples are homogenized in TRIzol® Reagent, GENEzol™ Reagent or other phenol, guanidine isothiocyanate reagents. Chloroform phase separation or isopropanol RNA precipitation is not required. Dnase I is included with this RNA kit for DNA sensitive downstream applications. Following sample homogenization, simply bind, wash and elute the high-quality, total RNA in RNase-free Water and use in a variety of downstream applications.
Advantages (Cat. # TRPD050, TRPD100, TRPD200)
- Purify total RNA in 10 minutes!
- No chlorophorm phase separation
- No isopropanol RNA precipitation
- No phenol carryover
- Compatibility: homogenize samples with GENEzol™, TRI-Reagent®, TRIzol®, RNAzol®, QIAzol® etc.
- Sample Volume: up to 200 μl of blood, buffy coat, serum, plasma), up to 5 x 106 cultured cells, 10-50 mg of tissue, 1 x 109 bacterial cells, 20-50 mg of plant tissue
- A cost effective phenol, guanidine isothiocyanate solution plus spin column system
- High quality RNA: A260/A280 >1.8, A260/A230 >1.8
- Binding Capacity: 50 µg RNA from ≥ 25 µl DNase/RNase-free Water
- Membrane: glass fiber membrane optimized for total RNA extraction
- Spin Columns: individually packaged RNA spin columns, certified RNase and DNase-free
- Storage: dry at room temperature (15-25ºC), DNase I can be shipped at room temperature but should be stored at -20ºC for extended periods
- cDNA Library Construction,
- Cloning,
- RT-PCR (Endpoint),
- Real-Time PCR,
- Nuclease Protection Assays,
- Northern Blotting
Components
- Pre-Wash Buffer
- DNase I
- DNase I Reaction Buffer
- Wash Buffer
- RNase-free Water
- RB Columns
- 2 ml Collection Tubes
Quality Control
The TriRNA Pure Kit is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. An Escherichia coli (1 × 109) culture (OD600=1.3, 1 ml) is harvested by centrifugation at 16,000 x g for 2 minutes, followed by GENEzol™ Reagent homogenization. RNA is then purified using a spin column procedure. 10 µl from a 50 µl eluate of purified RNA is analyzed by electrophoresis on a 0.8% agarose gel.
| Product Name | RXNS | Catalogue Number |
|---|---|---|
| GENEzol™ Reagent | 50/100/200 | GZR050/100/200 |
| GENEzol™ TriRNA Pure Kit | 50/100/200 | GZX050/100/200, GZXD050/100/200 |
| GENEzol™ TriRNA Bacteria Kit | 50/100 | GZB050/100 |
| TriRNA Pure Kit | 50/100/200 | TRP050/100/200, TRPD050/100/200 |
| RNA Cleanup Kit | 50/100 | PR050/100 |
| GENEzol™ 96 Well TriRNA Pure Kit | 4 x 96/10 x 96 | 96GZX04/10 |
