AccuGO Pfu DNA polymerase was designed to apply in the replication of DNA sequence required high fidelity and high efficiency. Like Pfu, AccuGO Pfu also exhibits 3’→5′ exonuclease activity that provides the proofreading ability and results in higher fidelity during DNA synthesis.
Moreover, AccuGO Pfu was engineered to have a better processivity than normal Pfu. Therefore, AccuGO Pfu can easily amplify up to 10 kb blunt-ended DNA fragment from variant template. Even if the template’s GC content is up to 70%, AccuGO Pfu still has good performance. In short, AccuGO Pfu DNA polymerase is an ideal tool for molecular cloning containing long or complicated amplicons.