GENEzol™ DNA Reagent Plant
Reagent DNA Extraction/Reagent DNA Extraction
GENEzol™ DNA Reagent Plant provides a quick and easy 3 step CTAB and chloroform based method to isolate total DNA (including genomic, mitochondrial and chloroplast DNA) from a variety of plant species (including algae and cyanobacteria). This unique reagent is able to lyse most common plant samples and plant samples with high a polysaccharide content. The extracted DNA is suitable for routine PCR screening, Real-Time PCR, Southern Blotting, Mapping and RFLP without phenol extraction.
Specifications (Cat. # GR100, GR200)
- Extract high molecular weight genomic DNA from a variety of plant species
- Sample Volume: up to 1 g of fresh plant tissue and up to 0.5 g of dry plant tissue
- A cost effective, scalable solution
- Format: simple and gentle CTAB and chloroform based DNA precipitation method
- Storage: dry at room temperature (15-25ºC) for up to 2 years, RNase should be stored at 4ºC for extended periods
- PCR,
- Real-Time PCR,
- Southern Blotting,
- Mapping,
- RFLP
Components
- GENEzol™ DNA Reagent Plant
- RNase A (50 mg/ml)
Quality Control
GENEzol™ DNA Reagent Plant is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. 50 mg of fresh Arabidopsis leaves are initially ground in GENEzol™ DNA Reagent Plant. A 15 µl aliquot of extracted genomic DNA from a 100 µl eluate is analyzed by electrophoresis on a 1% agarose gel.
Reagent Genomic DNA Extraction Kits Specifications
| Specifications | Geneaid™ DNA Isolation Kit Blood | Geneaid™ DNA Isolation Kit Cultured Cell | Geneaid™ DNA Isolation Kit Tissue | Geneaid™ DNA Isolation Kit Bacteria | Geneaid™ DNA Isolation Kit Yeast | GENEzol™ DNA Reagent Plant | ||
| Cat. # | GEB100 GEB01K GEB01K+ | GEC150 GEC1.5K GEC1.5K+ | GET150 GET1.5K GET1.5K+ | GEE150 GEE1.5K GEE1.5K+ | GEY100 GEY300 | GR100 GR200 | ||
| RXNS | 100-1,000 | 150-1,500 | 150-1,500 | 150-1,500 | 100-300 | 100-200 | ||
| Format | Reagent System | Reagent System | Reagent System | Reagent System | Reagent System | Reagent System | ||
| Sample | 300 µl-10 ml blood | up to 5x107 cultured cells | up to 200 mg tissue | up to 1.5x109 bacterial cells | up to 2x108 yeast cells | up to 1 g plant tissue | ||
| Elution Volume | 100 µl-1 ml | 50-200 µl | 100-200 µl | 100 µl | 50-100 µl | 50-100 µl | ||
| Operation Time | <40 minutes | <60 minutes | <70 minutes | <60 minutes | <40 minutes | <50 minutes |
| Tablo 1 | ||||
| Product Name | RXNS | Catalogue Number | ||
| Geneaid™ DNA Isolation Kit (Bacteria) | 150/1,500 | GEE100/01K/01K+ | ||
| Geneaid™ DNA Isolation Kit (Blood) | 100/1,000 | GEB100/01K/01K+ | ||
| Geneaid™ DNA Isolation Kit (Tissue) | 150/1,500 | GET150/1.5K/1.5K+ | ||
| Geneaid™ DNA Isolation Kit (Cultured Cell) | 150/1,500 | GEC150/1.5K/1.5K+ | ||
| Geneaid™ DNA Isolation Kit (Yeast) | 100/300 | GEY100/300 | ||
| GENEzol™ DNA Reagent Plant | 100/200 | GR100/200 | ||
