Geneaid™ DNA Isolation Kit (Bacteria)
Reagent DNA Extraction/Reagent DNA Extraction
The Geneaid™ DNA Isolation Kit (Bacteria) offers a simple and gentle reagent DNA precipitation method for isolating protein free, high molecular weight genomic and viral DNA from Gram (+) positive and Gram (-) negative bacteria cells, suitable for archiving or sensitive downstream applications. This highly versatile solution based system can be scaled proportionately in order to satisfy larger sample volumes providing a convenient sample-storage procedure with minimal hands on time. Initially cells are lysed in the presence of detergents and a proprietary DNA stabilization solution followed by RNase A treatment. Once proteins and other contaminants are removed DNA is precipitated then rehydrated. The high quality extracted DNA is ready for use in a variety of downstream applications.
Specifications (Cat. # GEE150, GEE1.5K, GEE1.5K+)
- Isolate high molecular weight DNA for archiving or sensitive downstream applications
- High yield, High quality DNA (A260/A280 = 1.8-2.0)
- Sample: 0.5-1.5 x 109 bacteria cells
- Easily scalable to satisfy larger bacteria sample volumes
- Minimal hands on time
- Convenient, simple and gentle reagent DNA precipitation method
- Storage: dry at room temperature (15-25°C) for up to 2 years, Lysozyme is shipped at room temperature and should be stored at -20°C for up to 1 year
- DNA archiving,
- PCR,
- AFLP,
- RFLP/PADP,
- Southern Blotting,
- Real-time PCR
Components
- Cell Lysis Buffer
- Gram+ Buffer
- Protein Removal Buffer
- DNA Hydration Buffer (10 mM Tris-HCL, 1 mM EDTA, pH8.0)
- RNase A (10 mg/ml) – not included with GEE1.5K
- Lysozyme – not included with GEE1.5K
Quality Control
The quality of the Geneaid™ DNA Isolation Kit (Bacteria) is tested on a lot-to-lot basis according to Geneaid’s ISO-certified quality management system. Genomic DNA is isolated from 0.5-1.5 x 109 bacteria cells. The isolated DNA (5-15 µg with an A260/A280 ratio of 1.8–2.0) is quantified with a spectrophotometer and analyzed by electrophoresis.
Reagent Genomic DNA Extraction Kits Specifications
| Specifications | Geneaid™ DNA Isolation Kit Blood | Geneaid™ DNA Isolation Kit Cultured Cell | Geneaid™ DNA Isolation Kit Tissue | Geneaid™ DNA Isolation Kit Bacteria | Geneaid™ DNA Isolation Kit Yeast | GENEzol™ DNA Reagent Plant | ||
| Cat. # | GEB100 GEB01K GEB01K+ | GEC150 GEC1.5K GEC1.5K+ | GET150 GET1.5K GET1.5K+ | GEE150 GEE1.5K GEE1.5K+ | GEY100 GEY300 | GR100 GR200 | ||
| RXNS | 100-1,000 | 150-1,500 | 150-1,500 | 150-1,500 | 100-300 | 100-200 | ||
| Format | Reagent System | Reagent System | Reagent System | Reagent System | Reagent System | Reagent System | ||
| Sample | 300 µl-10 ml blood | up to 5x107 cultured cells | up to 200 mg tissue | up to 1.5x109 bacterial cells | up to 2x108 yeast cells | up to 1 g plant tissue | ||
| Elution Volume | 100 µl-1 ml | 50-200 µl | 100-200 µl | 100 µl | 50-100 µl | 50-100 µl | ||
| Operation Time | <40 minutes | <60 minutes | <70 minutes | <60 minutes | <40 minutes | <50 minutes |
| Tablo 1 | ||||
| Product Name | RXNS | Catalogue Number | ||
| Geneaid™ DNA Isolation Kit (Bacteria) | 150/1,500 | GEE100/01K/01K+ | ||
| Geneaid™ DNA Isolation Kit (Blood) | 100/1,000 | GEB100/01K/01K+ | ||
| Geneaid™ DNA Isolation Kit (Tissue) | 150/1,500 | GET150/1.5K/1.5K+ | ||
| Geneaid™ DNA Isolation Kit (Cultured Cell) | 150/1,500 | GEC150/1.5K/1.5K+ | ||
| Geneaid™ DNA Isolation Kit (Yeast) | 100/300 | GEY100/300 | ||
| GENEzol™ DNA Reagent Plant | 100/200 | GR100/200 | ||
